Standard Curve (Sample)
Human Midnolin (MIDN) ELISA Kit | MIDN elisa kit
Human Midnolin (MIDN) ELISA Kit
Reactivity
Human
Synonyms
Midnolin (MIDN); N/A; Human Midnolin (MIDN) ELISA Kit; MIDN; DKFZp547M072; MIDN elisa kit
Reactivity
Human
Specificity
Human Midnolin (MIDN) ELISA Kit has high sensiti vity and excellent speci fici ty for detect ion o of Human MIDNMIDN. No signific ant cross cross-reactivity or interference between Human MIDN and analogues was observed.
Sequence Length
468
Assay Type
Sandwich
Samples
Human serum, cell culture su pernates and ot her bio logical fluids
Detection Range
50 pg/ml - 800 pg/ml
Sensitivity
< 5pg/ml ml
Intra-assay Precision
Four samples of known concentration were tested tw enty times o n one plate to assess intraintra-assay prec ision. The C V (%) (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested i n twenty separate assays to assess interinter-assay pre cision. Assa ys were per formed by at least three t echnicians using two lots of compon ents. The CV (%) ?11%.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for MIDN elisa kit
Background: MIDN (Midnolin) is a Protein Coding gene. GO annotations related to this gene include kinase binding. May be involved in regulation of genes related to neurogenesis in the nucleolus.
Principle of the Assay: Human Midnolin (MIDN) ELISA Kit employs a two-site sandwich ELISA to quantitate MIDN in samples. An antibody specific for MIDN has b een pre pre-coated onto a microplat microplate. Standards and samples are pipetted into the wells an d any MIDN prese nt is bound by the immobilized antibody. After removing any unbound substances substances, HRP -ConjugateConjugated Human MIDN detection anti body is added to the we lls. Fo llowing a wash to r emove any unbound HRP reagent, a Ch romogen solution is adde d to the wells and color dev elops in proportion to the amount of MIDN bound in the i nitial s tep. The color development is st opped and the inten sity of th e color is measured.
Principle of the Assay: Human Midnolin (MIDN) ELISA Kit employs a two-site sandwich ELISA to quantitate MIDN in samples. An antibody specific for MIDN has b een pre pre-coated onto a microplat microplate. Standards and samples are pipetted into the wells an d any MIDN prese nt is bound by the immobilized antibody. After removing any unbound substances substances, HRP -ConjugateConjugated Human MIDN detection anti body is added to the we lls. Fo llowing a wash to r emove any unbound HRP reagent, a Ch romogen solution is adde d to the wells and color dev elops in proportion to the amount of MIDN bound in the i nitial s tep. The color development is st opped and the inten sity of th e color is measured.
NCBI and Uniprot Product Information
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Product Notes
The Human MIDN midn (Catalog #AAA331774) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331774 ELISA Kit recognizes Human MIDN. It is sometimes possible for the material contained within the vial of "Midnolin (MIDN), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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