Standard Curve (Sample)
Human MIF ELISA Kit | MIF elisa kit
Human MIF Pre-Coated ELISA Kit
Reactivity
Human
Synonyms
MIF; N/A; Human MIF Pre-Coated ELISA Kit; MIF elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human MIF
Assay Type
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
<20pg/ml
Components
Anti-Human MIF Pre-coated 96-well strip microplate
Human MIF Standard
Human MIF Biotinylated antibody (100x)
Avidin-Biotin-Peroxidase Complex (100x)
Sample Diluent
Antibody Diluent
Avidin-Biotin-Peroxidase Diluent
Wash Buffer Concentrate (Powder for 1000mL)
Color Developing Reagent
Stop Solution
Plate Sealers
Human MIF Standard
Human MIF Biotinylated antibody (100x)
Avidin-Biotin-Peroxidase Complex (100x)
Sample Diluent
Antibody Diluent
Avidin-Biotin-Peroxidase Diluent
Wash Buffer Concentrate (Powder for 1000mL)
Color Developing Reagent
Stop Solution
Plate Sealers
Interference
No detectable cross-reactivity with other relevant proteins
Preparation and Storage
Store the unopened product at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for MIF elisa kit
Macrophage migration inhibitory factor (MIF) is a small secreted protein that can act as a pleiotropic pro-inflammatory cytokine, as well as an enzyme. MIF pro-inflammatory activity can be initiated by signaling through CD74 and CD44, resulting in the secretion of TNF-a, IL-1, IL-6, IL-8, and various MMPs. The enzymatic activity of MIF is characterized by its ability to act as a tautomerase, capable of catalyzing the keto-to-enol isomerization of keto-phenylpyruvate and L-dopachrome. It appears as though MIF catalytic activity is dependent upon a trimeric configuration and a free N-terminal proline residue. Human MIF Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure MIF with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for MIF. It contains E Coli-expressed recombinant MIF. The detection antibody is a biotinylated goat polyclonal antibody specific for MIF. The kit is analytically validated with ready to use reagents.
NCBI and Uniprot Product Information
UniProt Accession #
UniProt Protein Name
Macrophage migration inhibitory factor
UniProt Entry Name
I4AY87_HUMAN
Similar Products
Product Notes
The Human MIF (Catalog #AAA79417) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA79417 ELISA Kit recognizes Human MIF. It is sometimes possible for the material contained within the vial of "MIF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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