Standard Curve (Sample)
Human MIP-3alpha ELISA Kit | MIP-3alpha elisa kit
MIP-3alpha Human ELISA Kit
Gene Names
CCL20; MIP-3ALPHA
Reactivity
Human
Applications
ELISA
Synonyms
MIP-3alpha; N/A; MIP-3alpha Human ELISA Kit; Human MIP-3alpha; MIP-3alpha elisa kit
Reactivity
Human
Assay Type
Sandwich
Detection Range
15.6 pg/ml-1000 pg/ml
Sensitivity
< 1pg/ml
Applicable Applications for MIP-3alpha elisa kit
ELISA
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for MIP-3alpha elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MIP-3alpha polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MIP-3alpha polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MIP-3alpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MIP-3alpha can be calculated.
Background: Macrophage Inflammatory Protein-3 (MIP-3alpha), also known as Chemokine (C-C motif) ligand 20 (CCL20) or liver activation regulated chemokine (LARC), is a small cytokine belonging to the CC chemokine family. the CCL20 gene contains 4 exons and maps to chromosome 2q35-q36. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils. CCL20 is expressed in several tissues with highest expression observed in peripheral blood lymphocytes, lymph nodes, liver, appendix, and fetal lung and lower levels in thymus, testis, prostate and gut. CCL20 is implicated in the formation and function of mucosal lymphoid tissues via chemoattraction of lymphocytes and dendritic cells towards the epithelial cells surrounding these tissues. CCL20 elicits its effects on its target cells by binding and activating the chemokine receptor CCR6.
Background: Macrophage Inflammatory Protein-3 (MIP-3alpha), also known as Chemokine (C-C motif) ligand 20 (CCL20) or liver activation regulated chemokine (LARC), is a small cytokine belonging to the CC chemokine family. the CCL20 gene contains 4 exons and maps to chromosome 2q35-q36. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils. CCL20 is expressed in several tissues with highest expression observed in peripheral blood lymphocytes, lymph nodes, liver, appendix, and fetal lung and lower levels in thymus, testis, prostate and gut. CCL20 is implicated in the formation and function of mucosal lymphoid tissues via chemoattraction of lymphocytes and dendritic cells towards the epithelial cells surrounding these tissues. CCL20 elicits its effects on its target cells by binding and activating the chemokine receptor CCR6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
maacrophage inflammatory protein 3alpha
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 20
NCBI Official Symbol
CCL20
NCBI Official Synonym Symbols
MIP-3ALPHA
NCBI Protein Information
C-C motif chemokine 20; maacrophage inflammatory protein 3alpha
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Product Notes
The Human MIP-3alpha (Catalog #AAA58868) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA58868 ELISA Kit recognizes Human MIP-3alpha. AAA Biotech's MIP-3alpha can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the MIP-3alpha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIP-3alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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