Standard Curve (Sample)
(Fig.1. Human Mitochondrial uncoupling protein 2 (UCP2) Standard Curve.)
Human Mitochondrial Uncoupling Protein 2 (UCP2) ELISA Kit | UCP2 elisa kit
Human Mitochondrial Uncoupling Protein 2 (UCP2) ELISA Kit
Gene Names
UCP2; UCPH; BMIQ4; SLC25A8
Reactivity
Human
Synonyms
Mitochondrial Uncoupling Protein 2 (UCP2); N/A; Human Mitochondrial Uncoupling Protein 2 (UCP2) ELISA Kit; UCP2; BMIQ4; SLC25A8; UCPH; uncoupling protein 2; UCP2 elisa kit
Reactivity
Human
Specificity
Human Mitochondrial uncoupling protein 2 (UCP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human UCP2. No significant cross-reactivity or interference between Human UCP2 and analogues was observed.
Sequence Length
309
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Mitochondrial uncoupling protein 2 (UCP2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Mitochondrial uncoupling protein 2 (UCP2) Standard Curve.)
Related Product Information for UCP2 elisa kit
Description: This Human Mitochondrial uncoupling protein 2 (UCP2) ELISA Kit employs a two-site sandwich ELISA to quantitate UCP2 in samples. An antibody specific for UCP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UCP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UCP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UCP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H/OH- are not known. UCPs contain the three homologous protein domains of MACPs. Mitochondrial uncoupling protein 2 is expressed in many tissues, with the greatest expression in skeletal muscle. Although it was originally thought to play a role in nonshivering thermogenesis, obesity and diabetes, it now appears that the main function of UCP2 is the control of mitochondria-derived reactive oxygen species. Chromosomal order is 5'-UCP3-UCP2-3'.
Background: UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H/OH- are not known. UCPs contain the three homologous protein domains of MACPs. Mitochondrial uncoupling protein 2 is expressed in many tissues, with the greatest expression in skeletal muscle. Although it was originally thought to play a role in nonshivering thermogenesis, obesity and diabetes, it now appears that the main function of UCP2 is the control of mitochondria-derived reactive oxygen species. Chromosomal order is 5'-UCP3-UCP2-3'.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Mitochondrial uncoupling protein 2
NCBI Official Synonym Full Names
uncoupling protein 2
NCBI Official Symbol
UCP2
NCBI Official Synonym Symbols
UCPH; BMIQ4; SLC25A8
NCBI Protein Information
mitochondrial uncoupling protein 2
UniProt Protein Name
Mitochondrial uncoupling protein 2
UniProt Gene Name
UCP2
UniProt Synonym Gene Names
SLC25A8; UCP 2
UniProt Entry Name
UCP2_HUMAN
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Product Notes
The Human UCP2 ucp2 (Catalog #AAA330317) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330317 ELISA Kit recognizes Human UCP2. It is sometimes possible for the material contained within the vial of "Mitochondrial Uncoupling Protein 2 (UCP2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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