Standard Curve (Sample)
(Fig.1. Human Mitogen-activated protein kinase 13 (MAPK13) Standard Curve.)
Human Mitogen-Activated Protein Kinase 13 (MAPK13) ELISA Kit | MAPK13 elisa kit
Human Mitogen-Activated Protein Kinase 13 (MAPK13) ELISA Kit
Gene Names
MAPK13; SAPK4; PRKM13; MAPK 13; MAPK-13; p38delta
Reactivity
Human
Synonyms
Mitogen-Activated Protein Kinase 13 (MAPK13); N/A; Human Mitogen-Activated Protein Kinase 13 (MAPK13) ELISA Kit; MAPK13; MGC99536; PRKM13; SAPK4; p38delta; mitogen-activated protein kinase p38 delta; stress-activated protein kinase 4; MAPK13 elisa kit
Reactivity
Human
Specificity
Human Mitogen-activated protein kinase 13 (MAPK13) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAPK13. No significant cross-reactivity or interference between Human MAPK13 and analogues was observed.
Sequence Length
365
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Mitogen-activated protein kinase 13 (MAPK13) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Mitogen-activated protein kinase 13 (MAPK13) Standard Curve.)
Related Product Information for MAPK13 elisa kit
Description: This Human Mitogen-activated protein kinase 13 (MAPK13) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPK13 in samples. An antibody specific for MAPK13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAPK13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPK13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPK13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Mitogen Activated Protein Kinase 13 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and cellular stress. MAP kinase kinases 3, and 6 can phosphorylate and activate this kinase. Transcription factor ATF2, and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase.Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells.
Background: Mitogen Activated Protein Kinase 13 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and cellular stress. MAP kinase kinases 3, and 6 can phosphorylate and activate this kinase. Transcription factor ATF2, and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase.Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mitogen-activated protein kinase 13
NCBI Official Synonym Full Names
mitogen-activated protein kinase 13
NCBI Official Symbol
MAPK13
NCBI Official Synonym Symbols
SAPK4; PRKM13; MAPK 13; MAPK-13; p38delta
NCBI Protein Information
mitogen-activated protein kinase 13
UniProt Protein Name
Mitogen-activated protein kinase 13
UniProt Gene Name
MAPK13
UniProt Synonym Gene Names
PRKM13; SAPK4; MAP kinase 13; MAPK 13; MAP kinase p38 delta
UniProt Entry Name
MK13_HUMAN
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Product Notes
The Human MAPK13 mapk13 (Catalog #AAA331882) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331882 ELISA Kit recognizes Human MAPK13. It is sometimes possible for the material contained within the vial of "Mitogen-Activated Protein Kinase 13 (MAPK13), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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