Standard Curve (Sample)
(Fig.1. Human Myosin-IXb (MYO9B) Standard Curve.)
Human Myosin-IXb (MYO9B) ELISA Kit | MYO9B elisa kit
Human Myosin-IXb (MYO9B) ELISA Kit
Gene Names
MYO9B; MYR5; CELIAC4
Reactivity
Human
Synonyms
Myosin-IXb (MYO9B); N/A; Human Myosin-IXb (MYO9B) ELISA Kit; MYO9B; CELIAC4; MYR5; unconventional myosin IXb; MYO9B elisa kit
Reactivity
Human
Specificity
Human Myosin-IXb (MYO9B) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYO9B. No significant cross-reactivity or interference between Human MYO9B and analogues was observed.
Sequence Length
2157
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Myosin-IXb (MYO9B) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Myosin-IXb (MYO9B) Standard Curve.)
Related Product Information for MYO9B elisa kit
Description: This Human Myosin-IXb (MYO9B) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO9B in samples. An antibody specific for MYO9B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYO9B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO9B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO9B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MYO9B encodes a member of the myosin family of actin-based molecular motor heavy chain proteins. Myosin-IXb represents an unconventional myosin; it should not be confused with the conventional non-muscle myosin-9 (MYH9). Myosin-IXb has four IQ motifs located in the neck domain that bind calmodulin, which serves as a light chain. Myosin-IXb complex has a single-headed structure and exhibits processive movement on actin filaments toward the minus-end. Myosin-IXb also has rho-GTPase activity. Polymorphisms in MYO9B are associated with celiac disease and ulcerative colitis susceptibility. Multiple transcript variants encoding different isoforms have been found for MYO9B.
Background: MYO9B encodes a member of the myosin family of actin-based molecular motor heavy chain proteins. Myosin-IXb represents an unconventional myosin; it should not be confused with the conventional non-muscle myosin-9 (MYH9). Myosin-IXb has four IQ motifs located in the neck domain that bind calmodulin, which serves as a light chain. Myosin-IXb complex has a single-headed structure and exhibits processive movement on actin filaments toward the minus-end. Myosin-IXb also has rho-GTPase activity. Polymorphisms in MYO9B are associated with celiac disease and ulcerative colitis susceptibility. Multiple transcript variants encoding different isoforms have been found for MYO9B.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
unconventional myosin-IXb isoform 1
NCBI Official Synonym Full Names
myosin IXB
NCBI Official Symbol
MYO9B
NCBI Official Synonym Symbols
MYR5; CELIAC4
NCBI Protein Information
unconventional myosin-IXb
UniProt Protein Name
Unconventional myosin-IXb
UniProt Gene Name
MYO9B
UniProt Synonym Gene Names
MYR5
UniProt Entry Name
MYO9B_HUMAN
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Product Notes
The Human MYO9B myo9b (Catalog #AAA331573) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331573 ELISA Kit recognizes Human MYO9B. It is sometimes possible for the material contained within the vial of "Myosin-IXb (MYO9B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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