Standard Curve (Sample)
(Fig.1. Human Myosin light chain 5 (MYL5) Standard Curve.)
Human Myosin Light Chain 5 (MYL5) ELISA Kit | MYL5 elisa kit
Human Myosin Light Chain 5 (MYL5) ELISA Kit
Gene Names
MYL5; MYLC2
Reactivity
Human
Synonyms
Myosin Light Chain 5 (MYL5); N/A; Human Myosin Light Chain 5 (MYL5) ELISA Kit; MYL5; myosin regulatory light chain 5; myosin; light polypeptide 5; regulatory; superfast myosin regulatory light chain 2; MYL5 elisa kit
Reactivity
Human
Specificity
Human Myosin light chain 5 (MYL5) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYL5. No significant cross-reactivity or interference between Human MYL5 and analogues was observed.
Sequence Length
173
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Myosin light chain 5 (MYL5) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Myosin light chain 5 (MYL5) Standard Curve.)
Related Product Information for MYL5 elisa kit
Description: This Human Myosin light chain 5 (MYL5) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL5 in samples. An antibody specific for MYL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYL5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MYL5 encodes one of the myosin light chains, a component of the hexameric ATPase cellular motor protein myosin. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. Myosin light chain 5, one of the regulatory light chains, is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia.
Background: MYL5 encodes one of the myosin light chains, a component of the hexameric ATPase cellular motor protein myosin. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. Myosin light chain 5, one of the regulatory light chains, is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
myosin light chain 5 isoform 1
NCBI Official Synonym Full Names
myosin light chain 5
NCBI Official Symbol
MYL5
NCBI Official Synonym Symbols
MYLC2
NCBI Protein Information
myosin light chain 5
UniProt Protein Name
Myosin light chain 5
UniProt Gene Name
MYL5
UniProt Synonym Gene Names
MYLC2; MyLC-2
UniProt Entry Name
MYL5_HUMAN
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Product Notes
The Human MYL5 myl5 (Catalog #AAA331602) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331602 ELISA Kit recognizes Human MYL5. It is sometimes possible for the material contained within the vial of "Myosin Light Chain 5 (MYL5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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