Standard Curve (Sample)
(Fig.1. Human Myosin-Va (MYO5A) Standard Curve.)
Human Myosin-Va (MYO5A) ELISA Kit | MYO5A elisa kit
Human Myosin-Va (MYO5A) ELISA Kit
Gene Names
MYO5A; GS1; MYO5; MYH12; MYR12
Reactivity
Human
Synonyms
Myosin-Va (MYO5A); N/A; Human Myosin-Va (MYO5A) ELISA Kit; MYO5A; GS1; MYH12; MYO5; MYR12; dilute; myosin V; myosin VA; myosin; heavy polypeptide kinase; myoxin; MYO5A elisa kit
Reactivity
Human
Specificity
Human Myosin-Va (MYO5A) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYO5A. No significant cross-reactivity or interference between Human MYO5A and analogues was observed.
Sequence Length
1855
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Myosin-Va (MYO5A) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Myosin-Va (MYO5A) Standard Curve.)
Related Product Information for MYO5A elisa kit
Description: This Human Myosin-Va (MYO5A) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO5A in samples. An antibody specific for MYO5A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYO5A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO5A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO5A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MYO5A is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. Myosin-Va encoded by MYO5A is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined.
Background: MYO5A is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. Myosin-Va encoded by MYO5A is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
unconventional myosin-Va isoform 1
NCBI Official Synonym Full Names
myosin VA
NCBI Official Symbol
MYO5A
NCBI Official Synonym Symbols
GS1; MYO5; MYH12; MYR12
NCBI Protein Information
unconventional myosin-Va
UniProt Protein Name
Unconventional myosin-Va
UniProt Gene Name
MYO5A
UniProt Synonym Gene Names
MYH12
UniProt Entry Name
MYO5A_HUMAN
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Product Notes
The Human MYO5A myo5a (Catalog #AAA331578) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331578 ELISA Kit recognizes Human MYO5A. It is sometimes possible for the material contained within the vial of "Myosin-Va (MYO5A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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