Standard Curve (Sample)
(Fig.1. Human N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (PIGL) Standard Curve.)
Human N-Acetylglucosaminyl-Phosphatidylinositol De-N-Acetylase (PIGL) ELISA Kit | PIGL elisa kit
Human N-Acetylglucosaminyl-Phosphatidylinositol De-N-Acetylase (PIGL) ELISA Kit
Gene Names
PIGL; CHIME
Reactivity
Human
Synonyms
N-Acetylglucosaminyl-Phosphatidylinositol De-N-Acetylase (PIGL); N/A; Human N-Acetylglucosaminyl-Phosphatidylinositol De-N-Acetylase (PIGL) ELISA Kit; N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase; N-acetylglucosaminylphosphatidylinositol deacetylase; phosphatidylinositol glycan; class L; PIGL elisa kit
Reactivity
Human
Specificity
Human N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (PIGL) ELISA Kit has high sensitivity and excellent specificity for detection of Human PIGL. No significant cross-reactivity or interference between Human PIGL and analogues was observed.
Sequence Length
252
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (PIGL) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (PIGL) Standard Curve.)
Related Product Information for PIGL elisa kit
Description: This Human N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (PIGL) ELISA Kit employs a two-site sandwich ELISA to quantitate PIGL in samples. An antibody specific for PIGL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIGL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIGL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIGL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Glycosylphosphatidylinositol (GPI) is used as a membrane anchor by many eukaryotic cell surface proteins. The first step in GPI biosynthesis involves the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to phosphatidylinositol (PI). The second step is de-N-acetylation of GlcNAc-PI. PIGL encodes a deduced 252-amino acid protein that shares 77% identity with the rat protein. Using transfection into mammalian PIGL-deficient cells, Watanabe et al. (1999) demonstrated that S. cerevisiae Gpi12 is the ortholog of human and rat PIGL. Disruption of Gpi12 in yeast resulted in a lethal phenotype. Using purified, recombinant rat PIGL, Watanabe et al. (1999) demonstrated that PIGL has GlcNAc-PI de-N-acetylase activity in vitro, which is enhanced by metal ions.
Background: Glycosylphosphatidylinositol (GPI) is used as a membrane anchor by many eukaryotic cell surface proteins. The first step in GPI biosynthesis involves the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to phosphatidylinositol (PI). The second step is de-N-acetylation of GlcNAc-PI. PIGL encodes a deduced 252-amino acid protein that shares 77% identity with the rat protein. Using transfection into mammalian PIGL-deficient cells, Watanabe et al. (1999) demonstrated that S. cerevisiae Gpi12 is the ortholog of human and rat PIGL. Disruption of Gpi12 in yeast resulted in a lethal phenotype. Using purified, recombinant rat PIGL, Watanabe et al. (1999) demonstrated that PIGL has GlcNAc-PI de-N-acetylase activity in vitro, which is enhanced by metal ions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase
NCBI Official Synonym Full Names
phosphatidylinositol glycan anchor biosynthesis class L
NCBI Official Symbol
PIGL
NCBI Official Synonym Symbols
CHIME
NCBI Protein Information
N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase
UniProt Protein Name
N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase
UniProt Gene Name
PIGL
UniProt Synonym Gene Names
PIG-L
UniProt Entry Name
PIGL_HUMAN
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Product Notes
The Human PIGL pigl (Catalog #AAA331389) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331389 ELISA Kit recognizes Human PIGL. It is sometimes possible for the material contained within the vial of "N-Acetylglucosaminyl-Phosphatidylinositol De-N-Acetylase (PIGL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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