Standard Curve (Sample)
(Fig.1. Human N-acetylglutamate synthase, mitochondrial (NAGS) Standard Curve.)
Human N-Acetylglutamate Synthase, Mitochondrial (NAGS) ELISA Kit | NAGS elisa kit
Human N-Acetylglutamate Synthase, Mitochondrial (NAGS) ELISA Kit
Gene Names
NAGS; AGAS; ARGA
Reactivity
Human
Synonyms
N-Acetylglutamate Synthase, Mitochondrial (NAGS); N/A; Human N-Acetylglutamate Synthase, Mitochondrial (NAGS) ELISA Kit; NAGS; AGAS; ARGA; MGC133025; NAGS elisa kit
Reactivity
Human
Specificity
Human N-acetylglutamate synthase, mitochondrial (NAGS) ELISA Kit has high sensitivity and excellent specificity for detection of Human NAGS. No significant cross-reactivity or interference between Human NAGS and analogues was observed.
Sequence Length
534
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human N-acetylglutamate synthase, mitochondrial (NAGS) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human N-acetylglutamate synthase, mitochondrial (NAGS) Standard Curve.)
Related Product Information for NAGS elisa kit
Description: This Human N-acetylglutamate synthase, mitochondrial (NAGS) ELISA Kit employs a two-site sandwich ELISA to quantitate NAGS in samples. An antibody specific for NAGS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAGS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAGS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAGS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The N-acetylglutamate synthase gene encodes a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAG is a cofactor of carbamyl phosphate synthetase I (CPSI), the first enzyme of the urea cycle in mammals. NAGS may regulate ureagenesis by altering NAG availability and, thereby, CPSI activity. Deficiencies in N-acetylglutamate synthase have been associated with hyperammonemia.
Background: The N-acetylglutamate synthase gene encodes a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAG is a cofactor of carbamyl phosphate synthetase I (CPSI), the first enzyme of the urea cycle in mammals. NAGS may regulate ureagenesis by altering NAG availability and, thereby, CPSI activity. Deficiencies in N-acetylglutamate synthase have been associated with hyperammonemia.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
N-acetylglutamate synthase, mitochondrial
NCBI Official Synonym Full Names
N-acetylglutamate synthase
NCBI Official Symbol
NAGS
NCBI Official Synonym Symbols
AGAS; ARGA
NCBI Protein Information
N-acetylglutamate synthase, mitochondrial
UniProt Protein Name
N-acetylglutamate synthase, mitochondrial
UniProt Gene Name
NAGS
UniProt Entry Name
NAGS_HUMAN
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Product Notes
The Human NAGS nags (Catalog #AAA331550) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331550 ELISA Kit recognizes Human NAGS. It is sometimes possible for the material contained within the vial of "N-Acetylglutamate Synthase, Mitochondrial (NAGS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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