Human N-myc Downstream Regulated Gene 2 (NDRG2) ELISA Kit | NDRG2 elisa kit
Human N-myc Downstream Regulated Gene 2 (NDRG2) ELISA Kit
Gene Names
NDRG2; SYLD
Reactivity
Human
Synonyms
N-myc Downstream Regulated Gene 2 (NDRG2); N/A; Human N-myc Downstream Regulated Gene 2 (NDRG2) ELISA Kit; SYLD; N-myc downstream-regulated gene 2 protein; NDRG2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of NDRG2. No significant cross-reactivity or interference between NDRG2 and analogues was observed.
Sequence Length
367
Samples
Human tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
< 0.115ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NDRG2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NDRG2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Standard Curve (Sample)
Related Product Information for NDRG2 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of NDRG2 in human tissue homogenates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NDRG2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NDRG2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NDRG2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NDRG2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NDRG2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NDRG2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NDRG2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NDRG2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,297 Da
NCBI Official Full Name
protein NDRG2 isoform c
NCBI Official Synonym Full Names
NDRG family member 2
NCBI Official Symbol
NDRG2
NCBI Official Synonym Symbols
SYLD
NCBI Protein Information
protein NDRG2
UniProt Protein Name
Protein NDRG2
UniProt Gene Name
NDRG2
UniProt Synonym Gene Names
KIAA1248; SYLD
