Human Na-K-Cl Cotransporter 2 (NKCC2) ELISA Kit | NKCC2 elisa kit
Human Na-K-Cl Cotransporter 2 (NKCC2) ELISA Kit
Gene Names
SLC12A1; BSC1; NKCC2
Reactivity
Human
Synonyms
Na-K-Cl Cotransporter 2 (NKCC2); N/A; Human Na-K-Cl Cotransporter 2 (NKCC2) ELISA Kit; SLC12A1; NKCC2; Solute Carrier Family 12 Member 1; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; Kidney-specific Na-K-Cl symporter; NKCC2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of NKCC2. No significant cross-reactivity or interference between NKCC2 and analogues was observed.
Sequence Length
1099
Samples
Human tissue homogenates, cell lysates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
31.2-2000pg/mL
Sensitivity
< 13.8pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NKCC2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NKCC2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Standard Curve (Sample)
Related Product Information for NKCC2 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of NKCC2 in human tissue homogenates, cell lysates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NKCC2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NKCC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NKCC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NKCC2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NKCC2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NKCC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NKCC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NKCC2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
– Da
NCBI Official Full Name
solute carrier family 12 member 1 isoform A
NCBI Official Synonym Full Names
solute carrier family 12 member 1
NCBI Official Symbol
SLC12A1
NCBI Official Synonym Symbols
BSC1; NKCC2
NCBI Protein Information
solute carrier family 12 member 1
UniProt Protein Name
Solute carrier family 12 member 1
UniProt Gene Name
SLC12A1
UniProt Synonym Gene Names
NKCC2
