Standard Curve (Sample)
(Fig.1. Human Neuronal apoptosis inhibitory protein (NAIP) Standard Curve.)
Human Neuronal Apoptosis Inhibitory Protein (NAIP) ELISA Kit | NAIP elisa kit
Human Neuronal Apoptosis Inhibitory Protein (NAIP) ELISA Kit
Gene Names
NAIP; BIRC1; NLRB1; psiNAIP
Reactivity
Human
Synonyms
Neuronal Apoptosis Inhibitory Protein (NAIP); N/A; Human Neuronal Apoptosis Inhibitory Protein (NAIP) ELISA Kit; BIRC1; FLJ18088; FLJ42520; FLJ58811; NLRB1; psiNAIP; NLR family; BIR domain containing 1; OTTHUMP00000125255; baculoviral IAP repeat-containing 1; neuronal apoptosis inhibitory protein; nucleotide-bindi; NAIP elisa kit
Reactivity
Human
Specificity
Human Neuronal apoptosis inhibitory protein (NAIP) ELISA Kit has high sensitivity and excellent specificity for detection of Human NAIP. No significant cross-reactivity or interference between Human NAIP and analogues was observed.
Sequence Length
1347
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Neuronal apoptosis inhibitory protein (NAIP) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Neuronal apoptosis inhibitory protein (NAIP) Standard Curve.)
Related Product Information for NAIP elisa kit
Description: This Human Neuronal apoptosis inhibitory protein (NAIP) ELISA Kit employs a two-site sandwich ELISA to quantitate NAIP in samples. An antibody specific for NAIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: NAIP is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This copy of the gene is full length; additional copies with truncations and internal deletions are also present in this region of chromosome 5q13. It is thought that NAIP is a modifier of spinal muscular atrophy caused by mutations in a neighboring gene, SMN1. Neuronal apoptosis inhibitory protein encoded by NAIP contains regions of homology to two baculovirus inhibitor of apoptosis proteins, and it is able to suppress apoptosis induced by various signals. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
Background: NAIP is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This copy of the gene is full length; additional copies with truncations and internal deletions are also present in this region of chromosome 5q13. It is thought that NAIP is a modifier of spinal muscular atrophy caused by mutations in a neighboring gene, SMN1. Neuronal apoptosis inhibitory protein encoded by NAIP contains regions of homology to two baculovirus inhibitor of apoptosis proteins, and it is able to suppress apoptosis induced by various signals. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
NAIP protein
NCBI Official Synonym Full Names
NLR family apoptosis inhibitory protein
NCBI Official Symbol
NAIP
NCBI Official Synonym Symbols
BIRC1; NLRB1; psiNAIP
NCBI Protein Information
baculoviral IAP repeat-containing protein 1
UniProt Protein Name
Baculoviral IAP repeat-containing protein 1
UniProt Gene Name
NAIP
UniProt Synonym Gene Names
BIRC1
UniProt Entry Name
BIRC1_HUMAN
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Product Notes
The Human NAIP naip (Catalog #AAA331548) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331548 ELISA Kit recognizes Human NAIP. It is sometimes possible for the material contained within the vial of "Neuronal Apoptosis Inhibitory Protein (NAIP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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