Human Neuropeptide Y (NPY) ELISA Kit | NPY elisa kit

Human Neuropeptide Y (NPY) Mini Samples ELISA Kit

Cat. Num. AAA188720

• Gene Names: NPY; PYY4
• Reactivity: Human
• Applications: ELISA
• Synonyms: Neuropeptide Y (NPY); N/A; Human Neuropeptide Y (NPY) Mini Samples ELISA Kit; Neuropeptide Y; NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY; NPY elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Neuropeptide Y (NPY).; No significant cross-reactivity or interference between Mini Samples Neuropeptide Y (NPY) and analogues was observed.
• Sequence Length: 97
• Assay Type: Competitive Inhibition
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 24.69-2,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 9.59pg/mL

• Applicable Applications for NPY elisa kit: ELISA
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Neuropeptide Y (NPY) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Neuropeptide Y (NPY) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Neuropeptide Y (NPY) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Neuropeptide Y (NPY) and unlabeled Mini Samples Neuropeptide Y (NPY) (Standards or samples) with the pre-coated antibody specific to Mini Samples Neuropeptide Y (NPY). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Neuropeptide Y (NPY) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Neuropeptide Y (NPY) in the sample.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

NCBI and Uniprot Product Information

• NCBI GI #: 386984
• NCBI GeneID: 4852
• UniProt Accession #: P01303
• NCBI Official Full Name: neuropeptide Y, partial
• NCBI Official Synonym Full Names: neuropeptide Y
• NCBI Official Symbol: NPY
• NCBI Official Synonym Symbols: PYY4
• NCBI Protein Information: pro-neuropeptide Y
• UniProt Protein Name: Pro-neuropeptide Y
• UniProt Gene Name: NPY
• UniProt Synonym Gene Names: NPY; CPON
• UniProt Entry Name: NPY_HUMAN