Human Neurotrophin-3/NT-3 ELISA Kit | NT-3 elisa kit

Human Neurotrophin-3/NT-3 PicoKine Quick ELISA Kit

Cat. Num. AAA128058

• Gene Names: NTF3; NT3; HDNF; NGF2; NGF-2
• Reactivity: Human
• Applications: ELISA
• Synonyms: Neurotrophin-3/NT-3; N/A; Human Neurotrophin-3/NT-3 PicoKine Quick ELISA Kit; Neurotrophin-3; NT-3; HDNF; Nerve growth factor 2; NGF-2; Neurotrophic factor; NTF3; NT-3 elisa kit

• Reactivity: Human
• Samples: cell culture supernatants and serum
• Assay Range: 15.6pg/ml-1,000pg/ml
• Sensitivity: <2pg/ml
• Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Y139-T257
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for NT-3 elisa kit: ELISA
• Kit Components: Description/Quantity; Pre-coated 96-well strip microplate/1; Standard/2 vials, 10 ng/tube; Biotinylated antibody (100x)/100ul; Avidin-Biotin-Peroxidase Complex (100x)/100ul; Sample Diluent/30ml; Antibody Diluent/12ml; Avidin-Biotin-Peroxidase Diluent/12ml; Color Developing Reagent (TMB)/10ml; Stop Solution/10ml; Wash Buffer (25x)/20ml; Adhesive plate sealers/4
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Incubator.; Automated plate washer (optional).; Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.
• Sample Data:

  Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
  O.D.	0.014	0.065	0.173	0.248	0.477	0.761	1.270	1.886

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/ml)	30	147	434	31	141	418
  Standard deviation	1.83	6.02	29.94	2.32	7.61	33.44
  CV (%)	6.1%	4.1%	6.9%	7.5%	5.4%	8%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	30	28	27	28	28	1.08	3.8%
  Sample 2	147	147	172	166	158	11.2	7%
  Sample 3	434	515	474	436	464	33.1	7.1%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Standard Curve (Sample)

(Human Neurotrophin-3 PicoKine ELISA Kit standard curve)

Related Product Information for NT-3 elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for NT-3 elisa kit

Quick ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 156630995
• NCBI GeneID: 4908
• NCBI Accession #: NP_001096124.1
• NCBI GenBank Nucleotide #: NM_001102654.1
• UniProt Accession #: P20783; Q6FH50; B7Z1T5
• Molecular Weight: 29,355 Da
• NCBI Official Full Name: neurotrophin-3 isoform 1 preproprotein
• NCBI Official Synonym Full Names: neurotrophin 3
• NCBI Official Symbol: NTF3
• NCBI Official Synonym Symbols: NT3; HDNF; NGF2; NGF-2
• NCBI Protein Information: neurotrophin-3; NT-3; neurotrophic factor; nerve growth factor 2
• UniProt Protein Name: Neurotrophin-3
• UniProt Gene Name: NTF3
• UniProt Synonym Gene Names: NT-3; NGF-2
• UniProt Entry Name: NTF3_HUMAN