Standard Curve (Sample)
(Typical Standard Curve for NE ELISA (Sandwich))
Human Neutrophil Elastase (NE) ELISA Kit | NE elisa kit
Human Neutrophil Elastase (NE) Mini Samples ELISA Kit
Gene Names
ELANE; GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
Reactivity
Human
Synonyms
Neutrophil Elastase (NE); N/A; Human Neutrophil Elastase (NE) Mini Samples ELISA Kit; NE Mini-sample ELISA kit (Human); ELANE; HLE; HNE; ELA2; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Leukocyte Elastase; PMN Elastase; NE elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Neutrophil Elastase (NE). No significant cross-reactivity or interference between Mini Samples Neutrophil Elastase (NE) and analogues was observed.
Reactive with Human NE/Neutrophil Elastase
Reactive with Human NE/Neutrophil Elastase
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Method
Colormetric
Featured Series
ME kit
Detection Range
0.19-12ng/mL
Sensitivity
0.07ng/mL
Sample Size
15-25uL
Assay Time
3h
Function
Detects protein (micro-volume samples 15-25 ul)
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Neutrophil Elastase (NE) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Neutrophil Elastase (NE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Neutrophil Elastase (NE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Assay Procedure Summary
1. Prepare all reagents, samples and standards
2. Add 25ul standard or sample to each well. Incubate 1 hour at 37 degree C
3. Aspirate and add 25ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 25ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 25ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 20ul Stop Solution. Read at 450nm immediately.
2. Add 25ul standard or sample to each well. Incubate 1 hour at 37 degree C
3. Aspirate and add 25ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 25ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 25ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 20ul Stop Solution. Read at 450nm immediately.
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Neutrophil Elastase (NE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Neutrophil Elastase (NE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Neutrophil Elastase (NE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Neutrophil Elastase (NE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
Enzyme & Kinase, Infection immunity
Preparation and Storage
Store at 4 degree C for 1 month/-20 degree C for long-term (One year within shelf life)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
(Typical Standard Curve for NE ELISA (Sandwich))
Standard Curve (Sample)
(Typical Standard Curve for NE ELISA (Sandwich))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,518 Da
NCBI Official Full Name
neutrophil elastase preproprotein
NCBI Official Synonym Full Names
elastase, neutrophil expressed
NCBI Official Symbol
ELANE
NCBI Official Synonym Symbols
GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
NCBI Protein Information
neutrophil elastase; elastase-2; medullasin; PMN elastase; leukocyte elastase; elastase 2, neutrophil; human leukocyte elastase; polymorphonuclear elastase; bone marrow serine protease; granulocyte-derived elastase
UniProt Protein Name
Neutrophil elastase
UniProt Gene Name
ELANE
UniProt Synonym Gene Names
ELA2; HLE
UniProt Entry Name
ELNE_HUMAN
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Product Notes
The Human NE elane (Catalog #AAA161604) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA161604 ELISA Kit recognizes Human NE. It is sometimes possible for the material contained within the vial of "Neutrophil Elastase (NE), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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