Standard Curve (Sample)
Human Normetanephrine ELISA Kit
Normetanephrine (Plasma) ELISA
Reactivity
Human
Synonyms
Normetanephrine; N/A; Normetanephrine (Plasma) ELISA; Normetanephrine elisa kit
Reactivity
Human
Assay Type
Quantitative Competitive
Samples
Plasma
Design
Enzyme immunoassay (ELISA) technique.
Controls
2 controls, ready to use.
Assay Description
15 min. Centrifuge + 15 min. incubation (RT) + 15-20 hours (2-8 degree C) + 30 min. (RT) + 30 min. (RT) = 16-21 hours, 30 min.
Standards
6 calibrators, ready to use.
Sample Volume
500 uL
Preparation and Storage
2 - 8 degree C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Normetanephrine elisa kit
Background: Metanephrine and normetanephrine are the metabolites of the catecholamines epinephrine and norepinephrine, respectively [1]. Cells derived from neuroendocrine tumors (e.g. pheochromocytoma and paraganglioma) are known to produce catecholamines, which are secreted episodically via vesicles into the blood stream [2, 3]. But beside this, a small portion of the catecholamines is metabolized inside the tumor cells to the corresponding catecholamines metabolites - namely metanephrine, normetanephrine (and 3-methoxytyramine in the case of dopamine) - which are secreted at low levels continuously into the blood stream [4, 5].
Principle of the Assay: Enzyme Immunoassay for the quantitative determination of free normetanephrine in plasma. The determination of normetanephrine helps in the detection of paragangliomas and pheochromocytomas. Normetanephrine (normetadrenaline) is first extracted using an ion exchange matrix followed by an acylation process. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples compete with the solid phase bound analytes for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate resulting in a colour reaction. The reaction is monitored at a wavelength of 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations. Manual processing of the ELISA is recommended. The use of automatic laboratory equipment is the responsibility of the user. This product is not intended to clinical identifys.
Intended Uses: Enzyme Immunoassay for the quantitative determination of free normetanephrine in plasma. The determination of normetanephrine helps in the detection of paragangliomas and pheochromocytomas. Normetanephrine (normetadrenaline) is first extracted using an ion exchange matrix followed by an acylation process. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples compete with the solid phase bound analytes for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate resulting in a colour reaction. The reaction is monitored at a wavelength of 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations. Manual processing of the ELISA is recommended. The use of automatic laboratory equipment is the responsibility of the user. This product is not intended to clinical identifys.
Principle of the Assay: Enzyme Immunoassay for the quantitative determination of free normetanephrine in plasma. The determination of normetanephrine helps in the detection of paragangliomas and pheochromocytomas. Normetanephrine (normetadrenaline) is first extracted using an ion exchange matrix followed by an acylation process. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples compete with the solid phase bound analytes for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate resulting in a colour reaction. The reaction is monitored at a wavelength of 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations. Manual processing of the ELISA is recommended. The use of automatic laboratory equipment is the responsibility of the user. This product is not intended to clinical identifys.
Intended Uses: Enzyme Immunoassay for the quantitative determination of free normetanephrine in plasma. The determination of normetanephrine helps in the detection of paragangliomas and pheochromocytomas. Normetanephrine (normetadrenaline) is first extracted using an ion exchange matrix followed by an acylation process. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples compete with the solid phase bound analytes for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate resulting in a colour reaction. The reaction is monitored at a wavelength of 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations. Manual processing of the ELISA is recommended. The use of automatic laboratory equipment is the responsibility of the user. This product is not intended to clinical identifys.
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Product Notes
The Human Normetanephrine (Catalog #AAA72593) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA72593 ELISA Kit recognizes Human Normetanephrine. It is sometimes possible for the material contained within the vial of "Normetanephrine, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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