Human NOSTRIN (Nitric Oxide Synthase Traffic Inducer) ELISA Kit | NOSTRIN elisa kit

Human NOSTRIN (Nitric Oxide Synthase Traffic Inducer) ELISA Kit

Cat. Num. AAA27622

• Gene Names: NOSTRIN; DaIP2
• Reactivity: Human
• Synonyms: NOSTRIN (Nitric Oxide Synthase Traffic Inducer); N/A; Human NOSTRIN (Nitric Oxide Synthase Traffic Inducer) ELISA Kit; BM247 homolog; DaIP2; eNOS trafficking inducer; NOSTRIN; NOSTRIN elisa kit

• Reactivity: Human
• Specificity: Specifically recognize NOSTRIN, no obvious cross reaction with other analogues
• Sequence Length: 478
• Assay Type: Sandwich
• Samples: Serum, plasma, cell culture supernatant and other biological samples
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml

• Intra-assay Precision: Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
• Inter-assay Precision: Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for NOSTRIN elisa kit

Background: NOSTRIN (nitric oxide synthase trafficker isoform 1), also known as endothelial nitric oxide synthase traffic inducer, is a member of the Pombe Cdc15 homology (PCH) family of proteins. NOSTRIN is expressed in the vascular endothelial cells of highly vascularized tissues such as placenta, lung, kidney and heart. NOSTRIN typically exists as a trimer. It functions as an adaptor protein binding to caveolin-1 via an internal domain and NOS3 via its SH3 domain, forming a ternary complex which facilitates caveolar transport of NOS3. The NOS3 protein is responsible for the production of nitric oxide (NO), a potent mediator in various biological processes. The translocation of NOS3 from the plasma membrane to intracellular vesicle-like structures diminishes NO production. NOSTRIN also interacts with Dynamin and N-WASP via its SH3 domain.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti NOSTRIN antibody was precoated onto the 96-well plate. The biotin conjugated anti NOSTRIN antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with NOSTRIN conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of NOSTRIN in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

NCBI and Uniprot Product Information

• NCBI GI #: 284172483
• NCBI GeneID: 115677
• NCBI Accession #: NP_001165103.1
• NCBI GenBank Nucleotide #: NP_001165103.1
• UniProt Accession #: Q8IVI9
• NCBI Official Full Name: nostrin isoform 3
• NCBI Official Synonym Full Names: nitric oxide synthase trafficking
• NCBI Official Symbol: NOSTRIN
• NCBI Official Synonym Symbols: DaIP2
• NCBI Protein Information: nostrin
• UniProt Protein Name: Nostrin
• UniProt Gene Name: NOSTRIN
• UniProt Entry Name: NOSTN_HUMAN