Human Notch Homolog 2 (NOTCH2) ELISA Kit | NOTCH2 elisa kit

Human Notch Homolog 2 (NOTCH2) ELISA Kit

Cat. Num. AAA67697

• Gene Names: NOTCH2; hN2; AGS2; HJCYS
• Reactivity: Human
• Synonyms: Notch Homolog 2 (NOTCH2); N/A; Human Notch Homolog 2 (NOTCH2) ELISA Kit; AGS2 hN2 Neurogenic locus notch homolog protein 2; NOTCH2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NOTCH2. No significant cross-reactivity or interference between NOTCH2 and analogues was observed.
• Sequence Length: 1235
• Assay Type: Quantitative Sandwich
• Samples: Human tissue homogenates, cell lysates or other biological fluids
• Detection Range: 0.781-50ng/mL
• Sensitivity: < 0.33ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NOTCH2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NOTCH2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Standard Curve (Sample)

Related Product Information for NOTCH2 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NOTCH2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NOTCH2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NOTCH2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NOTCH2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of NOTCH2 in human tissue homogenates, cell lysates or other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 317008611
• NCBI GeneID: 4853
• NCBI Accession #: NP_001186930.1
• NCBI GenBank Nucleotide #: NM_001200001.1
• UniProt Accession #: Q04721; Q5T3X7; Q99734; Q9H240
• Molecular Weight: 265,405 Da
• NCBI Official Full Name: neurogenic locus notch homolog protein 2 isoform 2
• NCBI Official Synonym Full Names: notch 2
• NCBI Official Symbol: NOTCH2
• NCBI Official Synonym Symbols: hN2; AGS2; HJCYS
• NCBI Protein Information: neurogenic locus notch homolog protein 2
• UniProt Protein Name: Neurogenic locus notch homolog protein 2
• UniProt Gene Name: NOTCH2
• UniProt Synonym Gene Names: N2ICD