Standard Curve (Sample)
Human NOV/CCN3 ELISA Kit | NOV elisa kit
Human NOV/CCN3 PicoKine ELISA Kit
Gene Names
NOV; CCN3; NOVh; IBP-9; IGFBP9; IGFBP-9
Reactivity
Human
Synonyms
NOV/CCN3; N/A; Human NOV/CCN3 PicoKine ELISA Kit; Protein NOV homolog; nephroblastoma overexpressed; CCN family member 3; CCN3; IBP-9; IGF-binding protein 9; IGFBP-9; IGFBP9; Insulin-like growth factor-binding protein 9; Nephroblastoma overexpressed; Nephroblastoma overexpressed gene protein homolog; Nephroblastoma-overexpressed gene protein homolog; NOV; NOV_HUMAN; NovH; NOV elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human CCN3
Sequence Length
357
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<2 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for NOV elisa kit
Background: NOV (nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the diagnosis of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells.
Principle of the Assay: The Picokine™ Human CCN3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human CCN3 with a 96-well strip plate that is pre-coated with antibody specific for CCN3. The detection antibody is a biotinylated antibody specific for CCN3. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human CCN3 with immunogen: Expression system for standard: NSO; Immunogen sequence: T32-M357. The kit is analytically validated with ready-to-use reagents. To measure Human CCN3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human CCN3 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CCN3 in the sample.
Principle of the Assay: The Picokine™ Human CCN3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human CCN3 with a 96-well strip plate that is pre-coated with antibody specific for CCN3. The detection antibody is a biotinylated antibody specific for CCN3. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human CCN3 with immunogen: Expression system for standard: NSO; Immunogen sequence: T32-M357. The kit is analytically validated with ready-to-use reagents. To measure Human CCN3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human CCN3 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CCN3 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
39,162 Da
NCBI Official Full Name
Protein NOV homolog
NCBI Official Synonym Full Names
nephroblastoma overexpressed
NCBI Official Symbol
NOV
NCBI Official Synonym Symbols
CCN3; NOVh; IBP-9; IGFBP9; IGFBP-9
NCBI Protein Information
protein NOV homolog; CCN family member 3; IGF-binding protein 9; insulin-like growth factor-binding protein 9; nephroblastoma-overexpressed gene protein homolog
UniProt Protein Name
Protein NOV homolog
UniProt Gene Name
NOV
UniProt Synonym Gene Names
CCN3; IGFBP9; NOVH; NovH; IBP-9; IGF-binding protein 9; IGFBP-9
UniProt Entry Name
NOV_HUMAN
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Product Notes
The Human NOV nov (Catalog #AAA11627) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11627 ELISA Kit recognizes Human NOV. It is sometimes possible for the material contained within the vial of "NOV/CCN3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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