Standard Curve (Sample)
Human OPG (TNFRSF11B)/Osteoprotegerin ELISA Kit | OPG elisa kit
Human OPG (TNFRSF11B)/Osteoprotegerin ELISA Kit
Gene Names
TNFRSF11B; OPG; TR1; OCIF
Reactivity
Human
Synonyms
OPG (TNFRSF11B)/Osteoprotegerin; N/A; Human OPG (TNFRSF11B)/Osteoprotegerin ELISA Kit; Tumor necrosis factor receptor superfamily member 11B; tumor necrosis factor receptor superfamily, member 11b; MGC29565; MGC93568; OCIF; OPG; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TNFRSF 11B; TNFRSF11B; TR 1; TR1; TR11B_HUMAN; OPG elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human TNFRSF11B
Sequence Length
401
Samples
Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine
Detection Range
93.7 pg/ml - 6,000 pg/ml
Sensitivity
<5 pg/ml
Intra-assay Precision
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for OPG elisa kit
Background: Osteoprotegerin (OPG) is identical to osteoclastogenesis inhibitory factor (OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. OPG is considered to play an important role in the regulation of bone resorption by modifying osteoclast differentiation. Osteoprotegerin is an independent risk factor for the progression of atherosclerosis and onset of cardiovascular disease. It can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity. OPG system may play a critical role in the development of osteolytic bone disease in multiple myeloma and that targeting this system may have therapeutic potential. OPG also plays a role in the vascular system. The standard product used in this kit is recombinant OPG, a 55KDa glycoprotein.
Principle of the Assay: The Picokine™ Human TNFRSF11B Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human TNFRSF11B with a 96-well strip plate that is pre-coated with antibody specific for TNFRSF11B. The detection antibody is a biotinylated antibody specific for TNFRSF11B. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human TNFRSF11B with immunogen: Expression system for standard: NS0; Immunogen sequence: E22-L401. The kit is analytically validated with ready-to-use reagents. To measure Human TNFRSF11B, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human TNFRSF11B in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TNFRSF11B in the sample.
Principle of the Assay: The Picokine™ Human TNFRSF11B Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human TNFRSF11B with a 96-well strip plate that is pre-coated with antibody specific for TNFRSF11B. The detection antibody is a biotinylated antibody specific for TNFRSF11B. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human TNFRSF11B with immunogen: Expression system for standard: NS0; Immunogen sequence: E22-L401. The kit is analytically validated with ready-to-use reagents. To measure Human TNFRSF11B, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human TNFRSF11B in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TNFRSF11B in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
46,026 Da
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 11B
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 11b
NCBI Official Symbol
TNFRSF11B
NCBI Official Synonym Symbols
OPG; TR1; OCIF
NCBI Protein Information
tumor necrosis factor receptor superfamily member 11B; osteoprotegerin; osteoclastogenesis inhibitory factor
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 11B
UniProt Gene Name
TNFRSF11B
UniProt Synonym Gene Names
OCIF; OPG
UniProt Entry Name
TR11B_HUMAN
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Product Notes
The Human OPG tnfrsf11b (Catalog #AAA11548) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11548 ELISA Kit recognizes Human OPG. It is sometimes possible for the material contained within the vial of "OPG (TNFRSF11B)/Osteoprotegerin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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