Standard Curve (Sample)
Human Osteocalcin ELISA Kit | BGLAP elisa kit
Human Osteocalcin ELISA Kit
Gene Names
BGLAP; OC; BGP; OCN
Reactivity
Human
Synonyms
Osteocalcin; N/A; Human Osteocalcin ELISA Kit; BGLAP elisa kit
Reactivity
Human
Sequence Length
100
Assay Type
Sandwich
Samples
Human serum, cell culture supernates and other b iological fluids
Detection Range
75 ng/L - 1200 ng/L
Sensitivity
< 5.0 ng/L
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intraintra-assay precision. The CV (%) (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess interinter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%) (%)?11%.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for BGLAP elisa kit
Background: Osteocalcin (OC) is the most abundant noncollagenous protein in mature bone where it constitutes 1% to 2% of the total protein. Synthesized by osteoblasts, it is incorporated into the bon e matrix. Osteocalcin is a 49 49-residue protein with three gamma-carboxyglutamic acid residues, at positions 17, 21 and 24. These three residues confer on it a very strong ability to bind to hydroxyapatite. Osteocalcin is a noncollagenous protein found in bo ne and dentin. It is secreted by osteoblasts and thought to play a role in mineralization and calcium ion homeostasis. It has been stipulated that osteocalcin may also function as a negative regulator of bone formation, although its exact role is unknown.
Principle of the Assay: Human Osteocalcin ELISA Kit employs a two two-site sandwich ELISA to quantitate OC in samples. An antibody specific for OC has been pre pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OC present is bound by the immobilized antibody. After removing any unbound substances substances, HRPHRP-Conjugate Human OC detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proport ion to the amount of OC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: Human Osteocalcin ELISA Kit employs a two two-site sandwich ELISA to quantitate OC in samples. An antibody specific for OC has been pre pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OC present is bound by the immobilized antibody. After removing any unbound substances substances, HRPHRP-Conjugate Human OC detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proport ion to the amount of OC bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
osteocalcin preproprotein
NCBI Official Synonym Full Names
bone gamma-carboxyglutamate protein
NCBI Official Symbol
BGLAP
NCBI Official Synonym Symbols
OC; BGP; OCN
NCBI Protein Information
osteocalcin
UniProt Protein Name
Osteocalcin
UniProt Gene Name
BGLAP
UniProt Synonym Gene Names
BGP
UniProt Entry Name
OSTCN_HUMAN
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Product Notes
The Human BGLAP bglap (Catalog #AAA31505) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA31505 ELISA Kit recognizes Human BGLAP. It is sometimes possible for the material contained within the vial of "Osteocalcin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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