Reactivity
Human
Sequence Length
477
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids
Detection Range
10-4000pg/ml
Sensitivity
5.15pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Related Product Information for OTOL1 elisa kit
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human OTOL1 antibody. OTOL1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human OTOL1 Antibody is added and binds to OTOL1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated OTOL1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human OTOL1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Otolin 1 (also known as OTOL1) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Otolin 1 (also known as OTOL1) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,422 Da
NCBI Official Full Name
otolin-1
NCBI Official Synonym Full Names
otolin 1
NCBI Official Symbol
OTOL1
NCBI Official Synonym Symbols
C1QTNF15; C1QTNF16
NCBI Protein Information
otolin-1
UniProt Protein Name
Otolin-1
UniProt Gene Name
OTOL1