Human Oviductal Glycoprotein 1 (OVGP1) ELISA Kit | OVGP1 elisa kit

Human Oviductal Glycoprotein 1 (OVGP1) ELISA Kit

Cat. Num. AAA189790

• Gene Names: OVGP1; EGP; OGP; MUC9; CHIT5
• Reactivity: Human
• Synonyms: Oviductal Glycoprotein 1 (OVGP1); N/A; Human Oviductal Glycoprotein 1 (OVGP1) ELISA Kit; EGP; CHIT5; MUC9; OGP; Mucin 9; Oviductin; Oviduct-Specific Glycoprotein; Estrogen-dependent oviduct protein; OVGP1/Oviductal Glycoprotein 1; OVGP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of OVGP1. No significant cross-reactivity or interference between OVGP1 and analogues was observed.
• Assay Type: Quantitative Sandwich
• Samples: Human tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.117ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level OVGP1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level OVGP1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Store at 4 degree C for 1 month/-20 degree C for long-term (within shelf life); Shelf Life: 8 months; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for OVGP1 elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to OVGP1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to OVGP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain OVGP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of OVGP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of OVGP1 in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Product Categories/Family for OVGP1 elisa kit

Reproductive science

NCBI and Uniprot Product Information

• NCBI GI #: 58386720
• NCBI GeneID: 5016
• NCBI Accession #: NP_002548
• NCBI GenBank Nucleotide #: NM_002557.3
• UniProt Accession #: Q12889; Q15841; A0AV19; B9EGE1
• Molecular Weight: Predicted: 75 kDa; ; Observed: Due to extensive post-translational modification; OVGP1 migrates at ~140 kDa in SDS-PAGE.
• NCBI Official Full Name: oviduct-specific glycoprotein
• NCBI Official Synonym Full Names: oviductal glycoprotein 1, 120kDa
• NCBI Official Symbol: OVGP1
• NCBI Official Synonym Symbols: EGP; OGP; MUC9; CHIT5
• NCBI Protein Information: oviduct-specific glycoprotein; mucin 9; mucin-9; oviductin; oviduct glycoprotein; estrogen-dependent oviduct protein
• UniProt Protein Name: Oviduct-specific glycoprotein
• UniProt Gene Name: OVGP1
• UniProt Synonym Gene Names: MUC9; OGP
• UniProt Entry Name: OVGP1_HUMAN