Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Human P4HB ELISA Kit | P4HB elisa kit
Human P4HB ELISA Kit
Reactivity
Human
Synonyms
P4HB; N/A; Human P4HB ELISA Kit; P4HB ELISA Kit, Human; prolyl 4-hydroxylase, beta polypeptide; DSI ELISA Kit, Human; ERBA2L ELISA Kit, Human; GIT ELISA Kit, Human; P4Hbeta ELISA Kit, Human; PDI ELISA Kit, Human; PDIA1 ELISA Kit, Human; PHDB ELISA Kit, Human; PO4DB ELISA Kit, Human; PO4HB ELISA Kit, Human; PROHB ELISA Kit, Human; P4HB elisa kit
Reactivity
Human
Specificity
For the quantitative determination of Human P4HB concentration in cell lysate.
Assay Type
Solid Phase Sandwich ELISA (quantitative)
Sample Types
Lysate
Detection Range
125-8000pg/mL
Sensitivity
42.02pg/mL
Conjugation
HRP
Materials Provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Related Product Information for P4HB elisa kit
This P4HB ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human P4HB protein in Lysate. It contains recombinant Human P4HB, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Background: Protein disulfide-isomerase, also known as Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55 and P4HB, is a peripheral membrane protein that belongs to the protein disulfide isomerase family. P4HB is highly abundant. In some cell types, it seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. P4HB localizes near CD4-enriched regions on lymphoid cell surfaces. It is identified by mass spectrometry in melanosome fractions from stage I to stage IV. P4HB reduces and may activate fusogenic properties of HIV-1 gp12 surface protein, thereby enabling HIV-1 entry into the cell. P4HB catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. P4HB may therefore cause structural modifications of exofacial proteins. Inside the cell, it seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). P4HB may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts as a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
Background: Protein disulfide-isomerase, also known as Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55 and P4HB, is a peripheral membrane protein that belongs to the protein disulfide isomerase family. P4HB is highly abundant. In some cell types, it seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. P4HB localizes near CD4-enriched regions on lymphoid cell surfaces. It is identified by mass spectrometry in melanosome fractions from stage I to stage IV. P4HB reduces and may activate fusogenic properties of HIV-1 gp12 surface protein, thereby enabling HIV-1 entry into the cell. P4HB catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. P4HB may therefore cause structural modifications of exofacial proteins. Inside the cell, it seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). P4HB may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts as a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
References
Kivirikko KI, et al., 1989, FASEB J., 3 (5): 1609-17.
Pihlajaniemi T, et al., 1991, J Hepatol., 13, Suppl 3: S2
Fenouillet E., et al., 2001, J. Infect. Dis. 183:744-752.
Gevaert K., et al., 2003, Nat. Biotechnol. 21:566-569.
Barbouche R., et al., 2003, J. Biol. Chem. 278:3131-3136.
Pihlajaniemi T, et al., 1991, J Hepatol., 13, Suppl 3: S2
Fenouillet E., et al., 2001, J. Infect. Dis. 183:744-752.
Gevaert K., et al., 2003, Nat. Biotechnol. 21:566-569.
Barbouche R., et al., 2003, J. Biol. Chem. 278:3131-3136.
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Product Notes
The Human P4HB (Catalog #AAA258394) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA258394 ELISA Kit recognizes Human P4HB. It is sometimes possible for the material contained within the vial of "P4HB, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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