Standard Curve (Sample)
(Fig.1. Human Peptide chain release factor 1-like, mitochondrial (MTRF1L) Standard Curve.)
Human Peptide Chain Release Factor 1-Like, Mitochondrial (MTRF1L) ELISA Kit | MTRF1L elisa kit
Human Peptide Chain Release Factor 1-Like, Mitochondrial (MTRF1L) ELISA Kit
Gene Names
MTRF1L; MRF1L; HMRF1L; mtRF1a
Reactivity
Human
Synonyms
Peptide Chain Release Factor 1-Like, Mitochondrial (MTRF1L); N/A; Human Peptide Chain Release Factor 1-Like, Mitochondrial (MTRF1L) ELISA Kit; MTRF1L; RP1-101K10.1; MGC102748; MRF1L; mtRF1a; mitochondrial release factor 1 like; MTRF1L elisa kit
Reactivity
Human
Specificity
Human Peptide chain release factor 1-like, mitochondrial (MTRF1L) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTRF1L. No significant cross-reactivity or interference between Human MTRF1L and analogues was observed.
Sequence Length
380
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Peptide chain release factor 1-like, mitochondrial (MTRF1L) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Peptide chain release factor 1-like, mitochondrial (MTRF1L) Standard Curve.)
Related Product Information for MTRF1L elisa kit
Description: This Human Peptide chain release factor 1-like, mitochondrial (MTRF1L) ELISA Kit employs a two-site sandwich ELISA to quantitate MTRF1L in samples. An antibody specific for MTRF1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MTRF1L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTRF1L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTRF1L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Mitochondrial translational release factor 1-like encoded by MTRF1L plays a role in mitochondrial translation termination, and is thought to be a release factor that is involved in the dissociation of the complete protein from the final tRNA, the ribosome, and the cognate mRNA. Mitochondrial translational release factor 1-like acts upon UAA and UAG stop codons, but has no in vitro activity against UGA, which encodes tryptophan in human mitochondrion, or, the mitochondrial non-cognate stop codons, AGA and AGG. Mitochondrial translational release factor 1-like shares sequence similarity to bacterial release factors. Pseudogenes of this gene are found on chromosomes 4, 8, and 11. Alternative splicing results in multiple transcript variants.
Background: Mitochondrial translational release factor 1-like encoded by MTRF1L plays a role in mitochondrial translation termination, and is thought to be a release factor that is involved in the dissociation of the complete protein from the final tRNA, the ribosome, and the cognate mRNA. Mitochondrial translational release factor 1-like acts upon UAA and UAG stop codons, but has no in vitro activity against UGA, which encodes tryptophan in human mitochondrion, or, the mitochondrial non-cognate stop codons, AGA and AGG. Mitochondrial translational release factor 1-like shares sequence similarity to bacterial release factors. Pseudogenes of this gene are found on chromosomes 4, 8, and 11. Alternative splicing results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
peptide chain release factor 1-like, mitochondrial isoform 1
NCBI Official Synonym Full Names
mitochondrial translational release factor 1 like
NCBI Official Symbol
MTRF1L
NCBI Official Synonym Symbols
MRF1L; HMRF1L; mtRF1a
NCBI Protein Information
peptide chain release factor 1-like, mitochondrial
UniProt Protein Name
Peptide chain release factor 1-like, mitochondrial
UniProt Gene Name
MTRF1L
UniProt Synonym Gene Names
MTRF1A
UniProt Entry Name
RF1ML_HUMAN
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Product Notes
The Human MTRF1L mtrf1l (Catalog #AAA331637) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331637 ELISA Kit recognizes Human MTRF1L. It is sometimes possible for the material contained within the vial of "Peptide Chain Release Factor 1-Like, Mitochondrial (MTRF1L), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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