Standard Curve (Sample)
Human Peptidyl-prolyl cis-trans isomerase A, PPIase A ELISA Kit | PPIase A elisa kit
Human Peptidyl-prolyl cis-trans isomerase A, PPIase A ELISA Kit
Gene Names
PPIA; CYPA; CYPH; HEL-S-69p
Reactivity
Human
Synonyms
Peptidyl-prolyl cis-trans isomerase A, PPIase A; N/A; Human Peptidyl-prolyl cis-trans isomerase A, PPIase A ELISA Kit; 5.2.1.8, Peptidyl-prolyl cis-trans isomerase A (PPIase A); Rotamase A; Cyclophilin A; Cyclosporin A-binding protein; PPIA; PPIase A elisa kit
Reactivity
Human
Specificity
This assay recognizes recombinant and natural Human PPIase A. No significant cross-reactivity or interference was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between human PPIase A and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, plasma, tissue homogenates, cell culture supernate, and other biological fluids
Assay Type
Quantitative Competitive
Detection Range
0.78 - 50.0 ng/mL.
Preparation and Storage
Store at 4 degree C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for PPIase A elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human PPIase A concentrations in serum, plasma, tissue homogenates, cell culture supernate, and other biological fluids.
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. T he microtiter plate provided in this kit has been pre -coated with an antibody specific to PPIase A, During the reaction, PPIase A in the sample or standard competes with a fixed amount of biotin-labeled PPIase A for sites on a pre-coated Polyclonal antibody specific to PPIase A. Excess conjugate and unbound sample or standard are washed from the plate. Next, avidin-conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PPIase A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. T he microtiter plate provided in this kit has been pre -coated with an antibody specific to PPIase A, During the reaction, PPIase A in the sample or standard competes with a fixed amount of biotin-labeled PPIase A for sites on a pre-coated Polyclonal antibody specific to PPIase A. Excess conjugate and unbound sample or standard are washed from the plate. Next, avidin-conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PPIase A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,418 Da
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase A isoform 2
NCBI Official Synonym Full Names
peptidylprolyl isomerase A
NCBI Official Symbol
PPIA
NCBI Official Synonym Symbols
CYPA; CYPH; HEL-S-69p
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase A
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase A
UniProt Gene Name
PPIA
UniProt Synonym Gene Names
CYPA; PPIase A
Similar Products
Product Notes
The Human PPIase A ppia (Catalog #AAA310238) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA310238 ELISA Kit recognizes Human PPIase A. It is sometimes possible for the material contained within the vial of "Peptidyl-prolyl cis-trans isomerase A, PPIase A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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