Standard Curve (Sample)
(Fig.1. Human Pescadillo homolog (PES1) Standard Curve.)
Human Pescadillo Homolog (PES1) ELISA Kit | PES1 elisa kit
Human Pescadillo Homolog (PES1) ELISA Kit
Gene Names
PES1; PES; NOP7
Reactivity
Human
Synonyms
Pescadillo Homolog (PES1); N/A; Human Pescadillo Homolog (PES1) ELISA Kit; PES1; PES; pescadillo homolog 1; containing BRCT domain; PES1 elisa kit
Reactivity
Human
Specificity
Human Pescadillo homolog (PES1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PES1. No significant cross-reactivity or interference between Human PES1 and analogues was observed.
Sequence Length
588
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Pescadillo homolog (PES1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Pescadillo homolog (PES1) Standard Curve.)
Related Product Information for PES1 elisa kit
Description: This Human Pescadillo homolog (PES1) ELISA Kit employs a two-site sandwich ELISA to quantitate PES1 in samples. An antibody specific for PES1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PES1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PES1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PES1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PES1 is abnormally elevated in malignant tumors of astrocytic origin. It is a strongly conserved gene containing a BRCT domain that is essential for the activity of this gene product. The gene plays a crucial role in cell proliferation and may be necessary for oncogenic transformation and tumor progression.The deduced 588-amino acid PES1 protein is 74% identical to the zebrafish sequence. The authors noted the presence of a highly acidic region at the C terminus and that the protein has an alpha-helix structure. Immunoblot analysis showed that PES1 is expressed as an unmodified 72-kD protein and a SUMO1-modified 92-kD protein. In situ hybridization analysis revealed wide and high expression of Pes1 in embryonic mouse brain and spinal cord. Pes1 expression was upregulated in malignant, late-passage mouse astrocytes.
Background: PES1 is abnormally elevated in malignant tumors of astrocytic origin. It is a strongly conserved gene containing a BRCT domain that is essential for the activity of this gene product. The gene plays a crucial role in cell proliferation and may be necessary for oncogenic transformation and tumor progression.The deduced 588-amino acid PES1 protein is 74% identical to the zebrafish sequence. The authors noted the presence of a highly acidic region at the C terminus and that the protein has an alpha-helix structure. Immunoblot analysis showed that PES1 is expressed as an unmodified 72-kD protein and a SUMO1-modified 92-kD protein. In situ hybridization analysis revealed wide and high expression of Pes1 in embryonic mouse brain and spinal cord. Pes1 expression was upregulated in malignant, late-passage mouse astrocytes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pescadillo homolog isoform 1
NCBI Official Synonym Full Names
pescadillo ribosomal biogenesis factor 1
NCBI Official Symbol
PES1
NCBI Official Synonym Symbols
PES; NOP7
NCBI Protein Information
pescadillo homolog
UniProt Protein Name
Pescadillo homolog
UniProt Gene Name
PES1
UniProt Entry Name
PESC_HUMAN
Similar Products
Product Notes
The Human PES1 pes1 (Catalog #AAA331419) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331419 ELISA Kit recognizes Human PES1. It is sometimes possible for the material contained within the vial of "Pescadillo Homolog (PES1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.