Human Phosphatidylinositol 4-Kinase alpha (PI4KA) ELISA Kit | PI4KA elisa kit

Human Phosphatidylinositol 4-Kinase alpha (PI4KA) ELISA Kit

Cat. Num. AAA31489

• Gene Names: PI4KA; PIK4CA; PMGYCHA; pi4K230; PI4K-ALPHA
• Reactivity: Human
• Synonyms: Phosphatidylinositol 4-Kinase alpha (PI4KA); N/A; Human Phosphatidylinositol 4-Kinase alpha (PI4KA) ELISA Kit; PI4KA; FLJ16556; PI4K-ALPHA; PIK4CA; pi4K230; phosphatidylinositol 4-kinase 230; phosphatidylinositol 4-kinase type 3 alpha; phosphatidylinositol 4-kinase; typeI; alpha; PI4KA elisa kit

• Reactivity: Human
• Specificity: Human Phosphatidylinositol 4-kinase alpha (PI4KA) ELISA Kit has high sensitivity and excellent specificity for detection of Human PI4KA. No significant cross-reactivity or interference between Human PI4KA and analogues was observed.
• Sequence Length: 2102
• Assay Type: Sandwich
• Samples: Human serum, cell culture supernates and other biological fluids
• Detection Range: 7.5 pmol/L - 120 pmol/L
• Sensitivity: < 0.5pmol/L

• Intra-assay Precision: Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
• Inter-assay Precision: Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
• Preparation and Storage: Store at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for PI4KA elisa kit

Background: PI4Ka catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. Two transcript variants encoding different isoforms have been described for this gene.The predicted 854-amino acid protein has a calculated molecular mass of 97 kD. It contains an ankyrin repeat, a lipid kinase unique domain, a pleckstrin-homology domain, and a lipid kinase catalytic domain. PI4K-alpha has high amino acid sequence similarity to the yeast PI 4-kinases STT4 and PIK1; it has less amino acid sequence similarity to the catalytic domains of mammalian and yeast PI 3-kinases and to the yeast TOR family of proteins.

Principle of the Assay: Human Phosphatidylinositol 4-kinase alpha (PI4KA) ELISA Kit employs a two-site sandwich ELISA to quantitate PI4KA in samples. An antibody specific for PI4KA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PI4KA present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human PI4KA detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of PI4KA bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 348041302
• NCBI GeneID: 5297
• NCBI Accession #: NP_477352
• NCBI GenBank Nucleotide #: NP_477352.3
• UniProt Accession #: P42356
• NCBI Official Full Name: phosphatidylinositol 4-kinase alpha isoform 1
• NCBI Official Synonym Full Names: phosphatidylinositol 4-kinase alpha
• NCBI Official Symbol: PI4KA
• NCBI Official Synonym Symbols: PIK4CA; PMGYCHA; pi4K230; PI4K-ALPHA
• NCBI Protein Information: phosphatidylinositol 4-kinase alpha
• UniProt Protein Name: Phosphatidylinositol 4-kinase alpha
• UniProt Gene Name: PI4KA
• UniProt Synonym Gene Names: PIK4; PIK4CA; PI4-kinase alpha; PI4K-alpha; PtdIns-4-kinase alpha
• UniProt Entry Name: PI4KA_HUMAN