Human Phosphodiesterase 3A, cGMP Inhibited (PDE3A) ELISA Kit | PDE3A elisa kit

Human Phosphodiesterase 3A, cGMP Inhibited (PDE3A) ELISA Kit

Cat. Num. AAA68648

• Gene Names: PDE3A; HTNB; CGI-PDE; CGI-PDE A; CGI-PDE-A
• Reactivity: Human
• Synonyms: Phosphodiesterase 3A, cGMP Inhibited (PDE3A); N/A; Human Phosphodiesterase 3A, cGMP Inhibited (PDE3A) ELISA Kit; CGI-PDE; Cyclic GMP-inhibited phosphodiesterase A; cGMP-inhibited 3',5'-cyclic phosphodiesterase A; PDE3A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PDE3A. No significant cross-reactivity or interference between PDE3A and analogues was observed.
• Sequence Length: 1141
• Assay Type: Quantitative Sandwich
• Samples: Human tissue homogenates or other biological fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.101ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PDE3A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PDE3A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Standard Curve (Sample)

Related Product Information for PDE3A elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDE3A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PDE3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDE3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PDE3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PDE3A in human tissue homogenates or other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 70608155
• NCBI GeneID: 5139
• NCBI Accession #: NP_000912.3
• NCBI GenBank Nucleotide #: NM_000921.4
• UniProt Accession #: Q14432; O60865; Q13348; Q17RD1
• Molecular Weight: 124,979 Da
• NCBI Official Full Name: cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform 1
• NCBI Official Synonym Full Names: phosphodiesterase 3A
• NCBI Official Symbol: PDE3A
• NCBI Official Synonym Symbols: HTNB; CGI-PDE; CGI-PDE A; CGI-PDE-A
• NCBI Protein Information: cGMP-inhibited 3',5'-cyclic phosphodiesterase A
• UniProt Protein Name: cGMP-inhibited 3',5'-cyclic phosphodiesterase A
• UniProt Gene Name: PDE3A
• UniProt Synonym Gene Names: CGI-PDE A