Standard Curve (Sample)
(Fig.1. Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2) Standard Curve.)
Human Phosphoenolpyruvate Carboxykinase [GTP], Mitochondrial (PCK2) ELISA Kit | PCK2 elisa kit
Human Phosphoenolpyruvate Carboxykinase [GTP], Mitochondrial (PCK2) ELISA Kit
Gene Names
PCK2; PEPCK; PEPCK2; PEPCK-M
Reactivity
Human
Synonyms
Phosphoenolpyruvate Carboxykinase [GTP], Mitochondrial (PCK2); N/A; Human Phosphoenolpyruvate Carboxykinase [GTP], Mitochondrial (PCK2) ELISA Kit; PCK2; PEPCK; PEPCK-M; PEPCK2; PEP carboxykinase; mitochondrial phosphoenolpyruvate carboxykinase 2; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; PCK2 elisa kit
Reactivity
Human
Specificity
Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PCK2. No significant cross-reactivity or interference between Human PCK2 and analogues was observed.
Sequence Length
441
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2) Standard Curve.)
Related Product Information for PCK2 elisa kit
Description: This Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2) ELISA Kit employs a two-site sandwich ELISA to quantitate PCK2 in samples. An antibody specific for PCK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Phosphoenolpyruvate Carboxykinase 2,Mitochondrial is a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. PCK2 activation mediates an adaptive response to glucose depletion in lung cancer.
Background: Phosphoenolpyruvate Carboxykinase 2,Mitochondrial is a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. PCK2 activation mediates an adaptive response to glucose depletion in lung cancer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phosphoenolpyruvate carboxykinase
NCBI Official Synonym Full Names
phosphoenolpyruvate carboxykinase 2, mitochondrial
NCBI Official Symbol
PCK2
NCBI Official Synonym Symbols
PEPCK; PEPCK2; PEPCK-M
NCBI Protein Information
phosphoenolpyruvate carboxykinase [GTP], mitochondrial
UniProt Protein Name
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
UniProt Gene Name
PCK2
UniProt Synonym Gene Names
PEPCK2; PEPCK-M
UniProt Entry Name
PCKGM_HUMAN
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Product Notes
The Human PCK2 pck2 (Catalog #AAA331460) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331460 ELISA Kit recognizes Human PCK2. It is sometimes possible for the material contained within the vial of "Phosphoenolpyruvate Carboxykinase [GTP], Mitochondrial (PCK2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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