Standard Curve (Sample)
Human PINK1 (Serine/threonine-protein kinase PINK1, mitochondrial) ELISA Kit | PINK1 elisa kit
Human PINK1 (Serine/threonine-protein kinase PINK1, mitochondrial) ELISA Kit
Reactivity
Human
Synonyms
PINK1 (Serine/threonine-protein kinase PINK1; mitochondrial); Human PINK1 (Serine/threonine-protein kinase PINK1; mitochondrial) ELISA Kit; PINK1 elisa kit
Reactivity
Human
Specificity
Specifically recognize PINK1, no obvious cross reaction with other analogues
Sequence Length
766
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for PINK1 elisa kit
Background: PTEN induced putative kinase 1, PINK1, is a mitochondrial serine/threonine kinase involved in the normal function and integrity of mitochondria, as well as in reduction of cytochrome c release from mitochondria. PINK1 phosphorylates Parkin and promotes its translocation to mitochondria. Mutations in PINK1 are linked to autosomal recessive early onset Parkinson's disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of alpha- synuclein, as well as proteasome dysfunction.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti PINK1 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti PINK1 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with PINK1 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of PINK1 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti PINK1 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti PINK1 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with PINK1 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of PINK1 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
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Product Notes
The Human PINK1 pink1 (Catalog #AAA27605) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA27605 ELISA Kit recognizes Human PINK1. It is sometimes possible for the material contained within the vial of "PINK1 (Serine/threonine-protein kinase PINK1, mitochondrial), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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