Standard Curve (Sample)
(Fig.1. Human Platelet-derived growth factor D (PDGFD) Standard Curve.)
Human Platelet-Derived Growth Factor D (PDGFD) ELISA Kit | PDGFD elisa kit
Human Platelet-Derived Growth Factor D (PDGFD) ELISA Kit
Gene Names
PDGFD; IEGF; SCDGFB; MSTP036; SCDGF-B
Reactivity
Human
Synonyms
Platelet-Derived Growth Factor D (PDGFD); N/A; Human Platelet-Derived Growth Factor D (PDGFD) ELISA Kit; PDGFD; IEGF; MGC26867; MSTP036; SCDGF-B; SCDGFB; iris-expressed growth factor; spinal cord derived growth factor B; spinal cord-derived growth factor-B; PDGFD elisa kit
Reactivity
Human
Specificity
Human Platelet-derived growth factor D (PDGFD) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDGFD. No significant cross-reactivity or interference between Human PDGFD and analogues was observed.
Sequence Length
364
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Platelet-derived growth factor D (PDGFD) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Platelet-derived growth factor D (PDGFD) Standard Curve.)
Related Product Information for PDGFD elisa kit
Description: This Human Platelet-derived growth factor D (PDGFD) ELISA Kit employs a two-site sandwich ELISA to quantitate PDGFD in samples. An antibody specific for PDGFD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDGFD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGFD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGFD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Platelet-derived growth factor D is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene.
Background: Platelet-derived growth factor D is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
platelet-derived growth factor D isoform 2
NCBI Official Synonym Full Names
platelet derived growth factor D
NCBI Official Symbol
PDGFD
NCBI Official Synonym Symbols
IEGF; SCDGFB; MSTP036; SCDGF-B
NCBI Protein Information
platelet-derived growth factor D
UniProt Protein Name
Platelet-derived growth factor D
UniProt Gene Name
PDGFD
UniProt Synonym Gene Names
IEGF; SCDGFB; PDGF-D; SCDGF-B; PDGFD latent form
UniProt Entry Name
PDGFD_HUMAN
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Product Notes
The Human PDGFD pdgfd (Catalog #AAA331438) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331438 ELISA Kit recognizes Human PDGFD. It is sometimes possible for the material contained within the vial of "Platelet-Derived Growth Factor D (PDGFD), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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