Standard Curve (Sample)
(Fig.1. Human Platelet-derived growth factor subunit A (PDGFA) Standard Curve.)
Human Platelet-Derived Growth Factor Subunit A (PDGFA) ELISA Kit | PDGFA elisa kit
Human Platelet-Derived Growth Factor Subunit A (PDGFA) ELISA Kit
Gene Names
PDGFA; PDGF1; PDGF-A
Reactivity
Human
Synonyms
Platelet-Derived Growth Factor Subunit A (PDGFA); N/A; Human Platelet-Derived Growth Factor Subunit A (PDGFA) ELISA Kit; PDGFA; PDGF-A; PDGF1; PDGF A-chain; platelet-derived growth factor alpha; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha isoform 2 preproprotein; PDGFA elisa kit
Reactivity
Human
Specificity
Human Platelet-derived growth factor subunit A (PDGFA) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDGFA. No significant cross-reactivity or interference between Human PDGFA and analogues was observed.
Sequence Length
211
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Platelet-derived growth factor subunit A (PDGFA) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Platelet-derived growth factor subunit A (PDGFA) Standard Curve.)
Related Product Information for PDGFA elisa kit
Description: This Human Platelet-derived growth factor subunit A (PDGFA) ELISA Kit employs a two-site sandwich ELISA to quantitate PDGFA in samples. An antibody specific for PDGFA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDGFA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGFA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGFA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Platelet-derived growth factor subunit A is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene.
Background: Platelet-derived growth factor subunit A is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
platelet-derived growth factor subunit A isoform 1 preproprotein
NCBI Official Synonym Full Names
platelet derived growth factor subunit A
NCBI Official Symbol
PDGFA
NCBI Official Synonym Symbols
PDGF1; PDGF-A
NCBI Protein Information
platelet-derived growth factor subunit A
UniProt Protein Name
Platelet-derived growth factor subunit A
UniProt Gene Name
PDGFA
UniProt Synonym Gene Names
PDGF1; PDGF subunit A
UniProt Entry Name
PDGFA_HUMAN
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Product Notes
The Human PDGFA pdgfa (Catalog #AAA331965) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331965 ELISA Kit recognizes Human PDGFA. It is sometimes possible for the material contained within the vial of "Platelet-Derived Growth Factor Subunit A (PDGFA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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