Human Polypyrimidine Tract Binding Protein 1 (PTBP1) ELISA Kit | PTBP1 elisa kit

Human Polypyrimidine Tract Binding Protein 1 (PTBP1) ELISA Kit

Cat. Num. AAA93768

• Gene Names: PTBP1; PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I
• Reactivity: Human
• Synonyms: Polypyrimidine Tract Binding Protein 1 (PTBP1); N/A; Human Polypyrimidine Tract Binding Protein 1 (PTBP1) ELISA Kit; PTBP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PTBP1. No significant cross-reactivity or interference between PTBP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PTBP1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Intended Uses: This PLD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PLD. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for PTBP1 elisa kit

Principle of the Assay: PTBP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PTBP1 antibody and an PTBP1-HRP conjugate. The assay sample and buffer are incubated together with PTBP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTBP1 concentration since PTBP1 from samples and PTBP1-HRP conjugate compete for the anti-PTBP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PTBP1 from the sample, fewer sites are left to bind PTBP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTBP1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This PTBP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PTBP1. This ELISA kit for research use only, not for therapeutic or test applications!

NCBI and Uniprot Product Information

• NCBI GI #: 14165466
• NCBI GeneID: 5725
• NCBI Accession #: NP_114368.1
• NCBI GenBank Nucleotide #: NM_031991.3
• UniProt Accession #: P26599; Q9BUQ0
• Molecular Weight: 57,221 Da
• NCBI Official Full Name: polypyrimidine tract-binding protein 1 isoform c
• NCBI Official Synonym Full Names: polypyrimidine tract binding protein 1
• NCBI Official Symbol: PTBP1
• NCBI Official Synonym Symbols: PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I
• NCBI Protein Information: polypyrimidine tract-binding protein 1; hnRNP I; RNA-binding protein; 57 kDa RNA-binding protein PPTB-1; heterogeneous nuclear ribonucleoprotein I; heterogeneous nuclear ribonucleoprotein polypeptide I; polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I)
• UniProt Protein Name: Polypyrimidine tract-binding protein 1
• UniProt Gene Name: PTBP1
• UniProt Synonym Gene Names: PTB; PTB; hnRNP I
• UniProt Entry Name: PTBP1_HUMAN