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product-image-AAA245646_SC15.jpg Standard Curve (Sample)

Human Prefoldin subunit 4 (PFDN4) ELISA Kit | PFDN4 elisa kit

Human Prefoldin subunit 4 (PFDN4) ELISA Kit

Gene Names
PFDN4; C1; PFD4
Reactivity
Human
Synonyms
Prefoldin subunit 4 (PFDN4); N/A; Human Prefoldin subunit 4 (PFDN4) ELISA Kit; PFDN4 elisa kit
Ordering
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of PFDN4. No significant cross-reactivity or interference between PFDN4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PFDN4 and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.

Standard Curve (Sample)

product-image-AAA245646_SC15.jpg Standard Curve (Sample)
Related Product Information for PFDN4 elisa kit
Principle of the Assay: PFDN4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PFDN4 antibody and an PFDN4-HRP conjugate. The assay sample and buffer are incubated together with PFDN4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PFDN4 concentration since PFDN4 from samples and PFDN4-HRP conjugate compete for the anti-PFDN4 antibody binding site. Since the number of sites is limited, as more sites are occupied by PFDN4 from the sample, fewer sites are left to bind PFDN4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PFDN4 concentration in each sample is interpolated from this standard curve.

Intended Uses: This PFDN4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PFDN4. This ELISA kit for research use only, not for therapeutic or test applications!
Product Categories/Family for PFDN4 elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,314 Da
NCBI Official Full Name
prefoldin subunit 4
NCBI Official Synonym Full Names
prefoldin subunit 4
NCBI Official Symbol
PFDN4
NCBI Official Synonym Symbols
C1; PFD4
NCBI Protein Information
prefoldin subunit 4; prefoldin 4; protein C-1
UniProt Protein Name
Prefoldin subunit 4
UniProt Gene Name
PFDN4
UniProt Synonym Gene Names
PFD4
UniProt Entry Name
PFD4_HUMAN

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Product Notes

The Human PFDN4 pfdn4 (Catalog #AAA245646) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA245646 ELISA Kit recognizes Human PFDN4. It is sometimes possible for the material contained within the vial of "Prefoldin subunit 4 (PFDN4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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