Human Proenkephalin B (PDYN) ELISA Kit | PDYN elisa kit

Human Proenkephalin B (PDYN) ELISA Kit

Cat. Num. AAA27172

• Gene Names: PDYN; ADCA; PENKB; SCA23
• Reactivity: Human
• Synonyms: Proenkephalin B (PDYN); N/A; Human Proenkephalin B (PDYN) ELISA Kit; PDYN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PDYN. No significant cross-reactivity or interference between PDYN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PDYN and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for PDYN elisa kit

Principle of the Assay: PDYN ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PDYN antibody and an PDYN-HRP conjugate. The assay sample and buffer are incubated together with PDYN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDYN concentration since PDYN from samples and PDYN-HRP conjugate compete for the anti-PDYN antibody binding site. Since the number of sites is limited, as more sites are occupied by PDYN from the sample, fewer sites are left to bind PDYN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDYN concentration in each sample is interpolated from this standard curve.

Intended Uses: This PDYN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PDYN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for PDYN elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 300116234
• NCBI GeneID: 5173
• NCBI Accession #: NP_001177829.1
• NCBI GenBank Nucleotide #: NM_001190900.1
• Molecular Weight: 28,385 Da
• NCBI Official Full Name: proenkephalin-B preproprotein
• NCBI Official Synonym Full Names: prodynorphin
• NCBI Official Symbol: PDYN
• NCBI Official Synonym Symbols: ADCA; PENKB; SCA23
• NCBI Protein Information: proenkephalin-B; rimorphin; leumorphin; leu-enkephalin; preprodynorphin; preproenkephalin B; beta-neoendorphin-dynorphin; neoendorphin-dynorphin-enkephalin prepropeptide
• UniProt Protein Name: Proenkephalin-B
• UniProt Gene Name: PDYN
• UniProt Synonym Gene Names: Big Dyn; Dyn-A17; Dynorphin A; Dyn-B
• UniProt Entry Name: PDYN_HUMAN