Standard Curve (Sample)
(Fig.1. Human Prolactin-releasing hormone (PRH) Standard Curve.)
Human Prolactin-Releasing Hormone (PRH) ELISA Kit | PRH elisa kit
Human Prolactin-Releasing Hormone (PRH) ELISA Kit
Gene Names
PRLH; PRH; PRRP
Reactivity
Human
Synonyms
Prolactin-Releasing Hormone (PRH); N/A; Human Prolactin-Releasing Hormone (PRH) ELISA Kit; PRLH; PRH; PRRP; preproprolactin-releasing peptide; PRH elisa kit
Reactivity
Human
Specificity
Human Prolactin-releasing hormone (PRH) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRLH. No significant cross-reactivity or interference between Human PRLH and analogues was observed.
Sequence Length
87
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Prolactin-releasing hormone (PRH) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Prolactin-releasing hormone (PRH) Standard Curve.)
Related Product Information for PRH elisa kit
Description: This Human Prolactin-releasing hormone (PRH) ELISA Kit employs a two-site sandwich ELISA to quantitate PRLH in samples. An antibody specific for PRLH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRLH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRLH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRLH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Prolactin-releasing hormone, also known as PRLH, is a hypothetical human hormone or hormone releasing factor. Existence of this factor has been hypothesized as prolactin is the only currently known hormone for which almost exclusively negative regulating factors are known (such as dopamine, leukemia inhibitory factor, some prostaglandins) but few stimulating factors. While many prolactin stimulating and enhancing factors are well known (such as thyrotropin-releasing hormone, oxytocin, vasoactive intestinal peptide and estrogen) those have primary functions other than stimulating prolactin release and the search for hypothetical releasing factor or factors continues. The prolactin-releasing peptide identified in 1998 was a candidate for this function, however as of 2008 it appears its function is not yet completely elucidated.
Background: Prolactin-releasing hormone, also known as PRLH, is a hypothetical human hormone or hormone releasing factor. Existence of this factor has been hypothesized as prolactin is the only currently known hormone for which almost exclusively negative regulating factors are known (such as dopamine, leukemia inhibitory factor, some prostaglandins) but few stimulating factors. While many prolactin stimulating and enhancing factors are well known (such as thyrotropin-releasing hormone, oxytocin, vasoactive intestinal peptide and estrogen) those have primary functions other than stimulating prolactin release and the search for hypothetical releasing factor or factors continues. The prolactin-releasing peptide identified in 1998 was a candidate for this function, however as of 2008 it appears its function is not yet completely elucidated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
prolactin-releasing peptide
NCBI Official Synonym Full Names
prolactin releasing hormone
NCBI Official Symbol
PRLH
NCBI Official Synonym Symbols
PRH; PRRP
NCBI Protein Information
prolactin-releasing peptide
UniProt Protein Name
Prolactin-releasing peptide
UniProt Gene Name
PRLH
UniProt Synonym Gene Names
PRH; PrRP
UniProt Entry Name
PRRP_HUMAN
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Product Notes
The Human PRH prlh (Catalog #AAA331324) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331324 ELISA Kit recognizes Human PRH. It is sometimes possible for the material contained within the vial of "Prolactin-Releasing Hormone (PRH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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