Standard Curve (Sample)
(Fig.1. Human Proline dehydrogenase 1, mitochondrial (PRODH) Standard Curve.)
Human Proline Dehydrogenase 1, Mitochondrial (PRODH) ELISA Kit | PRODH elisa kit
Human Proline Dehydrogenase 1, Mitochondrial (PRODH) ELISA Kit
Gene Names
PRODH; POX; PIG6; HSPOX2; PRODH1; PRODH2; TP53I6
Reactivity
Human
Synonyms
Proline Dehydrogenase 1, Mitochondrial (PRODH); N/A; Human Proline Dehydrogenase 1, Mitochondrial (PRODH) ELISA Kit; PRODH; FLJ33744; HSPOX2; MGC148078; MGC148079; PIG6; POX; PRODH1; PRODH2; SCZD4; TP53I6; proline dehydrogenase 1; proline oxidase 2; proline oxidase; mitochondrial; tumor protein p53 inducible protein 6; PRODH elisa kit
Reactivity
Human
Specificity
Human Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRODH. No significant cross-reactivity or interference between Human PRODH and analogues was observed.
Sequence Length
600
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Proline dehydrogenase 1, mitochondrial (PRODH) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Proline dehydrogenase 1, mitochondrial (PRODH) Standard Curve.)
Related Product Information for PRODH elisa kit
Description: This Human Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit employs a two-site sandwich ELISA to quantitate PRODH in samples. An antibody specific for PRODH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRODH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRODH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRODH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PRODH encodes a mitochondrial protein that catalyzes the first step in proline degradation. Mutations in PRODH are associated with hyperprolinemia type 1 and susceptibility to schizophrenia 4 (SCZD4). PRODH is located on chromosome 22q11.21, a region which has also been associated with the contiguous gene deletion syndromes, DiGeorge and CATCH22. Alternatively spliced transcript variants encoding different isoforms have been found for PRODH.
Background: PRODH encodes a mitochondrial protein that catalyzes the first step in proline degradation. Mutations in PRODH are associated with hyperprolinemia type 1 and susceptibility to schizophrenia 4 (SCZD4). PRODH is located on chromosome 22q11.21, a region which has also been associated with the contiguous gene deletion syndromes, DiGeorge and CATCH22. Alternatively spliced transcript variants encoding different isoforms have been found for PRODH.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
proline dehydrogenase, mitochondrial
NCBI Official Synonym Full Names
proline dehydrogenase 1
NCBI Official Symbol
PRODH
NCBI Official Synonym Symbols
POX; PIG6; HSPOX2; PRODH1; PRODH2; TP53I6
NCBI Protein Information
proline dehydrogenase 1, mitochondrial
UniProt Protein Name
Proline dehydrogenase 1, mitochondrial
UniProt Gene Name
PRODH
UniProt Synonym Gene Names
PIG6; POX2
UniProt Entry Name
PROD_HUMAN
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Product Notes
The Human PRODH prodh (Catalog #AAA331315) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331315 ELISA Kit recognizes Human PRODH. It is sometimes possible for the material contained within the vial of "Proline Dehydrogenase 1, Mitochondrial (PRODH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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