Standard Curve (Sample)
(Fig.1. Human Proline-rich protein 4 (PRR4) Standard Curve.)
Human Proline-Rich Protein 4 (PRR4) ELISA Kit | PRR4 elisa kit
Human Proline-Rich Protein 4 (PRR4) ELISA Kit
Gene Names
PRR4; LPRP; PROL4
Reactivity
Human
Synonyms
Proline-Rich Protein 4 (PRR4); N/A; Human Proline-Rich Protein 4 (PRR4) ELISA Kit; PRR4; DKFZp779L1763; LPRP; PROL4; lacrimal proline rich protein; nasopharyngeal carcinoma-associated proline rich 4; proline-rich polypeptide 4; PRR4 elisa kit
Reactivity
Human
Specificity
Human Proline-rich protein 4 (PRR4) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRR4. No significant cross-reactivity or interference between Human PRR4 and analogues was observed.
Sequence Length
134
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Proline-rich protein 4 (PRR4) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Proline-rich protein 4 (PRR4) Standard Curve.)
Related Product Information for PRR4 elisa kit
Description: This Human Proline-rich protein 4 (PRR4) ELISA Kit employs a two-site sandwich ELISA to quantitate PRR4 in samples. An antibody specific for PRR4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRR4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRR4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRR4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Proline-rich protein 4 is a protein encoded by the PRR4 gene.Lacrimal proline rich protein is a member of the proline-rich protein family which lacks a conserved repetitive domain. It may have a role in protective functions in the eye.Two alternatively spliced transcript variants that encode different proteins have been described for this gene. The deduced 134-amino acid, 13.5-kD protein shares 45.5% sequence similarity with PRH1. It contains a 16-amino acid signal sequence, 21% proline residues, and an acidic N-terminal region. Southern blot analysis showed that LPRP is a single copy gene.Abundantly expressed in lacrimal gland where it is found in the acinar cells but not in the intralobular ducts. Also found in the submandibular gland, the parotid and sublingual glands.
Background: Proline-rich protein 4 is a protein encoded by the PRR4 gene.Lacrimal proline rich protein is a member of the proline-rich protein family which lacks a conserved repetitive domain. It may have a role in protective functions in the eye.Two alternatively spliced transcript variants that encode different proteins have been described for this gene. The deduced 134-amino acid, 13.5-kD protein shares 45.5% sequence similarity with PRH1. It contains a 16-amino acid signal sequence, 21% proline residues, and an acidic N-terminal region. Southern blot analysis showed that LPRP is a single copy gene.Abundantly expressed in lacrimal gland where it is found in the acinar cells but not in the intralobular ducts. Also found in the submandibular gland, the parotid and sublingual glands.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
proline-rich protein 4 isoform 2
NCBI Official Synonym Full Names
proline rich 4
NCBI Official Symbol
PRR4
NCBI Official Synonym Symbols
LPRP; PROL4
NCBI Protein Information
proline-rich protein 4
UniProt Protein Name
Proline-rich protein 4
UniProt Gene Name
PRR4
UniProt Synonym Gene Names
LPRP; PROL4
UniProt Entry Name
PROL4_HUMAN
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Product Notes
The Human PRR4 prr4 (Catalog #AAA331296) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331296 ELISA Kit recognizes Human PRR4. It is sometimes possible for the material contained within the vial of "Proline-Rich Protein 4 (PRR4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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