Standard Curve (Sample)
Human Prorelaxin H1 (Relaxin 1) ELISA Kit | RLN1 elisa kit
Human Prorelaxin H1 (Relaxin 1) ELISA Kit
Gene Names
RLN1; H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
Reactivity
Human
Synonyms
Prorelaxin H1 (Relaxin 1); N/A; Human Prorelaxin H1 (Relaxin 1) ELISA Kit; RLN1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human RLN1
Detection Range
78 pg/ml - 5,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in separate assays to assess inter- assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for RLN1 elisa kit
Background: Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to human chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix (cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.
Principle of the Assay: To measure Human RLN1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human RLN1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human RLN1 in the sample.
Intended Uses: This kit s a solid-phase immunoassay specially designed to measure Human RLN1 with a 96-well strip plate that is pre-coated with antibody specific for RLN1. The detection antibody is a biotinylated antibody specific for RLN1. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit includes Human RLN1 protein as standards.
Principle of the Assay: To measure Human RLN1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human RLN1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human RLN1 in the sample.
Intended Uses: This kit s a solid-phase immunoassay specially designed to measure Human RLN1 with a 96-well strip plate that is pre-coated with antibody specific for RLN1. The detection antibody is a biotinylated antibody specific for RLN1. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit includes Human RLN1 protein as standards.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,146 Da
NCBI Official Full Name
relaxin
NCBI Official Synonym Full Names
relaxin 1
NCBI Official Symbol
RLN1
NCBI Official Synonym Symbols
H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
NCBI Protein Information
prorelaxin H1; preprorelaxin H1
UniProt Protein Name
Prorelaxin H1
UniProt Gene Name
RLN1
UniProt Entry Name
REL1_HUMAN
Similar Products
Product Notes
The Human RLN1 rln1 (Catalog #AAA266527) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA266527 ELISA Kit recognizes Human RLN1. It is sometimes possible for the material contained within the vial of "Prorelaxin H1 (Relaxin 1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.