Standard Curve (Sample)
(Fig.1. Human Protein-arginine deiminase type-6 (PADI6) Standard Curve.)
Human Protein-Arginine Deiminase Type-6 (PADI6) ELISA Kit | PADI6 elisa kit
Human Protein-Arginine Deiminase Type-6 (PADI6) ELISA Kit
Gene Names
PADI6; hPADVI; PREMBL2
Reactivity
Human
Synonyms
Protein-Arginine Deiminase Type-6 (PADI6); N/A; Human Protein-Arginine Deiminase Type-6 (PADI6) ELISA Kit; PADI6; Peptidylarginine deiminase type 6; PADI6 elisa kit
Reactivity
Human
Specificity
Human Protein-arginine deiminase type-6 (PADI6) ELISA Kit has high sensitivity and excellent specificity for detection of Human PADI6. No significant cross-reactivity or interference between Human PADI6 and analogues was observed.
Sequence Length
694
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Protein-arginine deiminase type-6 (PADI6) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Protein-arginine deiminase type-6 (PADI6) Standard Curve.)
Related Product Information for PADI6 elisa kit
Description: This Human Protein-arginine deiminase type-6 (PADI6) ELISA Kit employs a two-site sandwich ELISA to quantitate PADI6 in samples. An antibody specific for PADI6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PADI6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PADI6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PADI6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Peptidylarginine deiminases (PADs) convert arginine residues in proteins into citrullines. Peptidylarginine deiminases (PADs, EC 3.5.3.15), including PADI6, make up a family of posttranslational protein modification enzymes that convert arginine residues to citrulline residues in the presence of calcium ions. PADI6,The deduced 694-amino acid protein has a calculated molecular mass of 77.7 kD. RT-PCR detected expression in adult human tissues, including ovary, peripheral blood leukocytes, and testis, with weaker expression in small intestine, spleen, lung, liver, and skeletal muscle. No expression was detected in other tissues examined. The order of the genes is p-telomere--PADI2--PADI1--PADI3--PADI4--PADI6. Mouse Padi6 maps to a syntenic Pad cluster on chromosome 4E1.
Background: Peptidylarginine deiminases (PADs) convert arginine residues in proteins into citrullines. Peptidylarginine deiminases (PADs, EC 3.5.3.15), including PADI6, make up a family of posttranslational protein modification enzymes that convert arginine residues to citrulline residues in the presence of calcium ions. PADI6,The deduced 694-amino acid protein has a calculated molecular mass of 77.7 kD. RT-PCR detected expression in adult human tissues, including ovary, peripheral blood leukocytes, and testis, with weaker expression in small intestine, spleen, lung, liver, and skeletal muscle. No expression was detected in other tissues examined. The order of the genes is p-telomere--PADI2--PADI1--PADI3--PADI4--PADI6. Mouse Padi6 maps to a syntenic Pad cluster on chromosome 4E1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein-arginine deiminase type-6
NCBI Official Synonym Full Names
peptidyl arginine deiminase 6
NCBI Official Symbol
PADI6
NCBI Official Synonym Symbols
hPADVI; PREMBL2
NCBI Protein Information
protein-arginine deiminase type-6
UniProt Protein Name
Protein-arginine deiminase type-6
UniProt Gene Name
PADI6
UniProt Synonym Gene Names
PAD6; hPADVI
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Product Notes
The Human PADI6 padi6 (Catalog #AAA331485) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331485 ELISA Kit recognizes Human PADI6. It is sometimes possible for the material contained within the vial of "Protein-Arginine Deiminase Type-6 (PADI6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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