Standard Curve (Sample)
(Fig.1. Human Protein disulfide-isomerase A2 (PDIA2) Standard Curve.)
Human Protein Disulfide-Isomerase A2 (PDIA2) ELISA Kit | PDIA2 elisa kit
Human Protein Disulfide-Isomerase A2 (PDIA2) ELISA Kit
Gene Names
PDIA2; PDI; PDA2; PDIP; PDIR
Reactivity
Human
Synonyms
Protein Disulfide-Isomerase A2 (PDIA2); N/A; Human Protein Disulfide-Isomerase A2 (PDIA2) ELISA Kit; PDIA2; LA16c-314G4.2; PDA2; PDI; PDIP; PDIR; Rho GDP dissociation inhibitor gamma; pancreatic protein disulfide isomerase; protein disulfide isomerase A2; protein disulfide isomerase; pancreatic; protein disul; PDIA2 elisa kit
Reactivity
Human
Specificity
Human Protein disulfide-isomerase A2 (PDIA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDIA2. No significant cross-reactivity or interference between Human PDIA2 and analogues was observed.
Sequence Length
525
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Protein disulfide-isomerase A2 (PDIA2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Protein disulfide-isomerase A2 (PDIA2) Standard Curve.)
Related Product Information for PDIA2 elisa kit
Description: This Human Protein disulfide-isomerase A2 (PDIA2) ELISA Kit employs a two-site sandwich ELISA to quantitate PDIA2 in samples. An antibody specific for PDIA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDIA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDIA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDIA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Protein disulfide isomerase or PDI is an enzyme in the endoplasmic reticulum in eukaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. Protein disulfide isomerases (EC 5.3.4.1), such as PDIP, are endoplasmic reticulum (ER) resident proteins that catalyze protein folding and thiol-disulfide interchange reactions. Desilva et al. (1996) cloned PDIP from an insulinoma subtraction cDNA library. The deduced 511-amino acid protein has a calculated molecular mass of about 56.6 kD and contains 2 thioredoxin-like catalytic sites, a C-terminal ER retention sequence (KEEL), and 3 potential N-glycosylation sites. PDIP shares about 46% identity with bovine, mouse, rabbit, and human PDIs. Northern blot analysis detected a 2.0-kb transcript expressed exclusively in pancreas.
Background: Protein disulfide isomerase or PDI is an enzyme in the endoplasmic reticulum in eukaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. Protein disulfide isomerases (EC 5.3.4.1), such as PDIP, are endoplasmic reticulum (ER) resident proteins that catalyze protein folding and thiol-disulfide interchange reactions. Desilva et al. (1996) cloned PDIP from an insulinoma subtraction cDNA library. The deduced 511-amino acid protein has a calculated molecular mass of about 56.6 kD and contains 2 thioredoxin-like catalytic sites, a C-terminal ER retention sequence (KEEL), and 3 potential N-glycosylation sites. PDIP shares about 46% identity with bovine, mouse, rabbit, and human PDIs. Northern blot analysis detected a 2.0-kb transcript expressed exclusively in pancreas.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein disulfide-isomerase A2
NCBI Official Synonym Full Names
protein disulfide isomerase family A member 2
NCBI Official Symbol
PDIA2
NCBI Official Synonym Symbols
PDI; PDA2; PDIP; PDIR
NCBI Protein Information
protein disulfide-isomerase A2
UniProt Protein Name
Protein disulfide-isomerase A2
UniProt Gene Name
PDIA2
UniProt Synonym Gene Names
PDIP; PDIp
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Product Notes
The Human PDIA2 pdia2 (Catalog #AAA331433) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331433 ELISA Kit recognizes Human PDIA2. It is sometimes possible for the material contained within the vial of "Protein Disulfide-Isomerase A2 (PDIA2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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