Human Protein Kinase C-Binding Protein NELL1 (NELL1) ELISA Kit | NELL1 elisa kit

Human Protein Kinase C-Binding Protein NELL1 (NELL1) ELISA Kit

Cat. Num. AAA219784

• Gene Names: NELL1; NRP1; IDH3GL
• Reactivity: Human
• Synonyms: Protein Kinase C-Binding Protein NELL1 (NELL1); N/A; Human Protein Kinase C-Binding Protein NELL1 (NELL1) ELISA Kit; NEL-like protein 1; Nel-related protein 1; NELL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NELL1. No significant cross-reactivity or interference between NELL1 and analogues was observed.
• Assay Type: Quantitative Sandwich
• Samples: Human serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.105ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NELL1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NELL1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Shelf Life: 12 months

Standard Curve (Sample)

Related Product Information for NELL1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NELL1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NELL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NELL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NELL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of NELL1 in human serum, plasma, tissue homogenates and other biological fluids.

Product Categories/Family for NELL1 elisa kit

Traditional ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 222352164
• NCBI GeneID: 4745
• NCBI Accession #: NP_006148.2
• NCBI GenBank Nucleotide #: NM_006157.3
• UniProt Accession #: Q92832; Q4VB90; Q4VB91; Q6NSY8; Q9Y472; B2CKC1
• Molecular Weight: 810
• NCBI Official Full Name: protein kinase C-binding protein NELL1 isoform 1
• NCBI Official Synonym Full Names: NEL-like 1 (chicken)
• NCBI Official Symbol: NELL1
• NCBI Official Synonym Symbols: NRP1; IDH3GL
• NCBI Protein Information: protein kinase C-binding protein NELL1; NEL-like protein 1; nel-related protein 1; NELL-1 protein short isoform; neural epidermal growth factor-like 1
• UniProt Protein Name: Protein kinase C-binding protein NELL1
• UniProt Gene Name: NELL1
• UniProt Synonym Gene Names: NRP1
• UniProt Entry Name: NELL1_HUMAN