Standard Curve (Sample)
(Fig.1. Human Protein phosphatase Slingshot homolog 2 (SSH2) Standard Curve.)
Human Protein Phosphatase Slingshot Homolog 2 (SSH2) ELISA Kit | SSH2 elisa kit
Human Protein Phosphatase Slingshot Homolog 2 (SSH2) ELISA Kit
Gene Names
SSH2; SSH-2; SSH-2L
Reactivity
Human
Synonyms
Protein Phosphatase Slingshot Homolog 2 (SSH2); N/A; Human Protein Phosphatase Slingshot Homolog 2 (SSH2) ELISA Kit; SSH2; KIAA1725; MGC78588; SSH-2; slingshot 2; SSH2 elisa kit
Reactivity
Human
Specificity
Human Protein phosphatase Slingshot homolog 2 (SSH2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SSH2. No significant cross-reactivity or interference between Human SSH2 and analogues was observed.
Sequence Length
195
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Protein phosphatase Slingshot homolog 2 (SSH2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Protein phosphatase Slingshot homolog 2 (SSH2) Standard Curve.)
Related Product Information for SSH2 elisa kit
Description: This Human Protein phosphatase Slingshot homolog 2 (SSH2) ELISA Kit employs a two-site sandwich ELISA to quantitate SSH2 in samples. An antibody specific for SSH2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SSH2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SSH2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SSH2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Two of the human proteins, SSH1L and SSH2, were enzymatically active when reacted with an artificial substrate, p-nitrophenyl phosphate. SSH3 did not exhibit activity toward p-nitrophenyl phosphate, and its expression did not reduce the level of P-cofilin in COS-7 cells. In mammalian cells, SSH1L and SSH2 suppressed LIMK1-induced actin reorganization. Furthermore, ssh, SSH1L, and SSH2 dephosphorylated P-cofilin in cultured cells and in cell-free assays. SSH1 encodes 3 isoforms, SSH1L, SSH1S, and SSH1B, and SSH2 encodes 2 isoforms, SSH2 and SSH2B. The SSH3 protein has 471 amino acids. Besides the catalytic domain, 2 other domains are conserved between Drosophila ssh and the human SSHs (domains A and B) and are unique to the SSH family.
Background: Two of the human proteins, SSH1L and SSH2, were enzymatically active when reacted with an artificial substrate, p-nitrophenyl phosphate. SSH3 did not exhibit activity toward p-nitrophenyl phosphate, and its expression did not reduce the level of P-cofilin in COS-7 cells. In mammalian cells, SSH1L and SSH2 suppressed LIMK1-induced actin reorganization. Furthermore, ssh, SSH1L, and SSH2 dephosphorylated P-cofilin in cultured cells and in cell-free assays. SSH1 encodes 3 isoforms, SSH1L, SSH1S, and SSH1B, and SSH2 encodes 2 isoforms, SSH2 and SSH2B. The SSH3 protein has 471 amino acids. Besides the catalytic domain, 2 other domains are conserved between Drosophila ssh and the human SSHs (domains A and B) and are unique to the SSH family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
SSH2 protein
NCBI Official Synonym Full Names
slingshot protein phosphatase 2
NCBI Official Symbol
SSH2
NCBI Official Synonym Symbols
SSH-2; SSH-2L
NCBI Protein Information
protein phosphatase Slingshot homolog 2
UniProt Protein Name
Protein phosphatase Slingshot homolog 2
UniProt Gene Name
SSH2
UniProt Synonym Gene Names
KIAA1725; SSH2L; SSH-2L; hSSH-2L
UniProt Entry Name
SSH2_HUMAN
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Product Notes
The Human SSH2 ssh2 (Catalog #AAA330583) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330583 ELISA Kit recognizes Human SSH2. It is sometimes possible for the material contained within the vial of "Protein Phosphatase Slingshot Homolog 2 (SSH2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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