Standard Curve (Sample)
(Fig.1. Human Protein Wnt-2b (WNT2B) Standard Curve.)
Human Protein Wnt-2b (WNT2B) ELISA Kit | WNT2B elisa kit
Human Protein Wnt-2b (WNT2B) ELISA Kit
Gene Names
WNT2B; WNT13
Reactivity
Human
Synonyms
Protein Wnt-2b (WNT2B); N/A; Human Protein Wnt-2b (WNT2B) ELISA Kit; WNT2B; WNT13; XWNT2; Xenopus; homolog of; wingless-type MMTV integration site family; member 13; WNT2B elisa kit
Reactivity
Human
Specificity
Human Protein Wnt-2b (WNT2B) ELISA Kit has high sensitivity and excellent specificity for detection of Human WNT2B. No significant cross-reactivity or interference between Human WNT2B and analogues was observed.
Sequence Length
391
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Protein Wnt-2b (WNT2B) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Protein Wnt-2b (WNT2B) Standard Curve.)
Related Product Information for WNT2B elisa kit
Description: This Human Protein Wnt-2b (WNT2B) ELISA Kit employs a two-site sandwich ELISA to quantitate WNT2B in samples. An antibody specific for WNT2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT2B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: WNT2B encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as human carcinogenesis. This gene produces two alternative transcript variants.WNT13 encodes a 372-amino acid polypeptide including a putative secretory signal peptide. Northern blot analysis revealed that WNT13 was expressed as a 2.5-kb mRNA in fetal brain, lung, and kidney. Unlike most other WNTs, which tend to have restricted expression in adult tissues, its transcript was found in a wide variety of adult tissues.
Background: WNT2B encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as human carcinogenesis. This gene produces two alternative transcript variants.WNT13 encodes a 372-amino acid polypeptide including a putative secretory signal peptide. Northern blot analysis revealed that WNT13 was expressed as a 2.5-kb mRNA in fetal brain, lung, and kidney. Unlike most other WNTs, which tend to have restricted expression in adult tissues, its transcript was found in a wide variety of adult tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Protein Wnt-2b
NCBI Official Synonym Full Names
Wnt family member 2B
NCBI Official Symbol
WNT2B
NCBI Official Synonym Symbols
WNT13
NCBI Protein Information
protein Wnt-2b
UniProt Protein Name
Protein Wnt-2b
UniProt Gene Name
WNT2B
UniProt Synonym Gene Names
WNT13
UniProt Entry Name
WNT2B_HUMAN
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Product Notes
The Human WNT2B wnt2b (Catalog #AAA330254) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330254 ELISA Kit recognizes Human WNT2B. It is sometimes possible for the material contained within the vial of "Protein Wnt-2b (WNT2B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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