Standard Curve (Sample)
(Fig.1. Human Proto-oncogene Wnt-3 (WNT3) Standard Curve.)
Human Proto-Oncogene Wnt-3 (WNT3) ELISA Kit | WNT3 elisa kit
Human Proto-Oncogene Wnt-3 (WNT3) ELISA Kit
Gene Names
WNT3; INT4; TETAMS
Reactivity
Human
Synonyms
Proto-Oncogene Wnt-3 (WNT3); N/A; Human Proto-Oncogene Wnt-3 (WNT3) ELISA Kit; WNT3; INT4; MGC131950; MGC138321; MGC138323; WNT-3 proto-oncogene protein; WNT3 elisa kit
Reactivity
Human
Specificity
Human Proto-oncogene Wnt-3 (WNT3) ELISA Kit has high sensitivity and excellent specificity for detection of Human WNT3. No significant cross-reactivity or interference between Human WNT3 and analogues was observed.
Sequence Length
355
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Proto-oncogene Wnt-3 (WNT3) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Proto-oncogene Wnt-3 (WNT3) Standard Curve.)
Related Product Information for WNT3 elisa kit
Description: This Human Proto-oncogene Wnt-3 (WNT3) ELISA Kit employs a two-site sandwich ELISA to quantitate WNT3 in samples. An antibody specific for WNT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT3 is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region.
Background: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT3 is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
WNT3
NCBI Official Synonym Full Names
Wnt family member 3
NCBI Official Symbol
WNT3
NCBI Official Synonym Symbols
INT4; TETAMS
NCBI Protein Information
proto-oncogene Wnt-3
UniProt Protein Name
Proto-oncogene Wnt-3
UniProt Gene Name
WNT3
UniProt Synonym Gene Names
INT4
UniProt Entry Name
WNT3_HUMAN
Similar Products
Product Notes
The Human WNT3 wnt3 (Catalog #AAA330253) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330253 ELISA Kit recognizes Human WNT3. It is sometimes possible for the material contained within the vial of "Proto-Oncogene Wnt-3 (WNT3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.