Standard Curve (Sample)
Human PTH1R ELISA Kit | PTH1R elisa kit
Human PTH1R PicoKine ELISA Kit
Gene Names
PTH1R; PFE; EKNS; PTHR; PTHR1
Reactivity
Human
Synonyms
PTH1R; N/A; Human PTH1R PicoKine ELISA Kit; Human PTH1R; parathyroid hormone 1 receptor; Parathyroid hormone/parathyroid hormone-related peptide receptor; PTH/PTHrP type I receptor; PTH/PTHr receptor; Parathyroid hormone 1 receptor; PTH1 receptor; PTHR; PTHR1; PTH1R elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human PTH1R
Sequence Length
593
Samples
Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for PTH1R elisa kit
Principle of the Assay: The Picokine™ Human PTH1R Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human PTH1R with a 96-well strip plate that is pre-coated with antibody specific for PTH1R. The detection antibody is a biotinylated antibody specific for PTH1R. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human PTH1R with immunogen: Expression system for standard: CHO, Immunogen sequence: Y23-M189. The kit is analytically validated with ready to use reagents. To measure Human PTH1R, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human PTH1R in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human PTH1R in the sample.!!Background/Introduction: Parathyroid hormone/parathyroid hormone-related peptide receptor, also known as parathyroid hormone 1 receptor (PTH1R), is a protein that in humans is encoded by the PTH1R gene. It is mapped to 3p21.31. The protein encoded by this gene is a member of the G-protein coupled receptor family 2. This protein is a receptor for parathyroid hormone (PTH) and for parathyroid hormone-like hormone (PTHLH). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. Defects in this receptor are known to be the cause of Jansen's metaphyseal chondrodysplasia (JMC), chondrodysplasia Blomstrand type (BOCD), as well as enchodromatosis. Two transcript variants encoding the same protein have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
parathyroid hormone/parathyroid hormone-related peptide receptor
NCBI Official Synonym Full Names
parathyroid hormone 1 receptor
NCBI Official Symbol
PTH1R
NCBI Official Synonym Symbols
PFE; EKNS; PTHR; PTHR1
NCBI Protein Information
parathyroid hormone/parathyroid hormone-related peptide receptor
UniProt Protein Name
Parathyroid hormone/parathyroid hormone-related peptide receptor
UniProt Gene Name
PTH1R
UniProt Synonym Gene Names
PTHR; PTHR1; PTH/PTHr receptor
UniProt Entry Name
PTH1R_HUMAN
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Product Notes
The Human PTH1R pth1r (Catalog #AAA125293) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA125293 ELISA Kit recognizes Human PTH1R. It is sometimes possible for the material contained within the vial of "PTH1R, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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