Standard Curve (Sample)
(Fig.1. Human Putative 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (PRHOXNB) Standard Curve.)
Human Putative 2-Oxo-4-Hydroxy-4-Carboxy-5-Ureidoimidazoline Decarboxylase (PRHOXNB) ELISA Kit | PRHOXNB elisa kit
Human Putative 2-Oxo-4-Hydroxy-4-Carboxy-5-Ureidoimidazoline Decarboxylase (PRHOXNB) ELISA Kit
Gene Names
URAD; PRHOXNB
Reactivity
Human
Synonyms
Putative 2-Oxo-4-Hydroxy-4-Carboxy-5-Ureidoimidazoline Decarboxylase (PRHOXNB); N/A; Human Putative 2-Oxo-4-Hydroxy-4-Carboxy-5-Ureidoimidazoline Decarboxylase (PRHOXNB) ELISA Kit; PRHOXNB; URAD; Parahox Cluster Neighbor; PRHOXNB elisa kit
Reactivity
Human
Specificity
Human Putative 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (PRHOXNB) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRHOXNB. No significant cross-reactivity or interference between Human PRHOXNB and analogues was observed.
Sequence Length
173
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Putative 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (PRHOXNB) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Putative 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (PRHOXNB) Standard Curve.)
Related Product Information for PRHOXNB elisa kit
Description: This Human Putative 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (PRHOXNB) ELISA Kit employs a two-site sandwich ELISA to quantitate PRHOXNB in samples. An antibody specific for PRHOXNB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRHOXNB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRHOXNB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRHOXNB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PRHOXNB belongs to the OHCU decarboxylase family. Recombinant TTL from Arabidopsis thaliana catalyzes two enzymatic reactions leading to the stereoselective formation of S-allantoin, hydrolysis of hydroxyisourate through a C-terminal Urah domain, and decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline through an N-terminal Urad domain. TTL originated in green algae through a Urad-Urah fusion, which entrapped an N-terminal PTS2 between the two domains. The presence of this gene in all Viridiplantae indicates that S-allantoin biosynthesis has general significance in plant nitrogen metabolism, while conservation of alternative splicing suggests that this mechanism has general implications in the regulation of the ureide pathway in flowering plants.
Background: PRHOXNB belongs to the OHCU decarboxylase family. Recombinant TTL from Arabidopsis thaliana catalyzes two enzymatic reactions leading to the stereoselective formation of S-allantoin, hydrolysis of hydroxyisourate through a C-terminal Urah domain, and decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline through an N-terminal Urad domain. TTL originated in green algae through a Urad-Urah fusion, which entrapped an N-terminal PTS2 between the two domains. The presence of this gene in all Viridiplantae indicates that S-allantoin biosynthesis has general significance in plant nitrogen metabolism, while conservation of alternative splicing suggests that this mechanism has general implications in the regulation of the ureide pathway in flowering plants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
putative 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase
NCBI Official Synonym Full Names
ureidoimidazoline (2-oxo-4-hydroxy-4-carboxy-5-) decarboxylase
NCBI Official Symbol
URAD
NCBI Official Synonym Symbols
PRHOXNB
NCBI Protein Information
putative 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase
UniProt Protein Name
Putative 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase
UniProt Gene Name
URAD
UniProt Synonym Gene Names
PRHOXNB; OHCU decarboxylase
UniProt Entry Name
URAD_HUMAN
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Product Notes
The Human PRHOXNB urad (Catalog #AAA331343) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331343 ELISA Kit recognizes Human PRHOXNB. It is sometimes possible for the material contained within the vial of "Putative 2-Oxo-4-Hydroxy-4-Carboxy-5-Ureidoimidazoline Decarboxylase (PRHOXNB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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