Human RBP4/Retinol Binding Protein 4 ELISA Kit | RBP4 elisa kit

Human RBP4/Retinol Binding Protein 4 PicoKine Fast ELISA Kit

Cat. Num. AAA125347

• Gene Names: RBP4; RDCCAS; MCOPCB10
• Reactivity: Human
• Synonyms: RBP4/Retinol Binding Protein 4; N/A; Human RBP4/Retinol Binding Protein 4 PicoKine Fast ELISA Kit; Human RBP4; Retinol-binding protein 4; Plasma retinol-binding protein; PRBP; RBP; Plasma retinol-binding protein(1-182); Plasma retinol-binding protein(1-181); Plasma retinol-binding protein(1-179); Plasma retinol-binding protein(1-176); RBP4; PRO2222; RBP4 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human RBP4
• Sequence Length: 201
• Samples: Cell culture supernates, serum, plasma(heparin, EDTA) and urine
• Detection Range: 312pg/ml-20000pg/ml
• Sensitivity: <10pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

Related Product Information for RBP4 elisa kit

Principle of the Assay: The Picokine™ Human RBP4 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human RBP4 with a 96-well strip plate that is pre-coated with antibody specific for RBP4. The detection antibody is a biotinylated antibody specific for RBP4. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human RBP4 with immunogen: Expression system for standard: NSO; Immunogen sequence: E19-L201. The kit is analytically validated with ready to use reagents. To measure Human RBP4, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human RBP4 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human RBP4 in the sample.!!Background/Introduction: Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol(vitamin A alcohol) in the blood. It is protein that in humans is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c(Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.

NCBI and Uniprot Product Information

• NCBI GI #: 55743122
• NCBI GeneID: 5950
• NCBI Accession #: NP_006735.2
• NCBI GenBank Nucleotide #: NP_006735.2
• UniProt Accession #: P02753
• NCBI Official Full Name: retinol-binding protein 4 isoform a
• NCBI Official Synonym Full Names: retinol binding protein 4
• NCBI Official Symbol: RBP4
• NCBI Official Synonym Symbols: RDCCAS; MCOPCB10
• NCBI Protein Information: retinol-binding protein 4
• UniProt Protein Name: Retinol-binding protein 4
• UniProt Gene Name: RBP4
• UniProt Synonym Gene Names: PRBP; RBP
• UniProt Entry Name: RET4_HUMAN